BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
植物组织培养技术即植物无菌培养技术，又称离体培养技术，是根据植物细胞具有全能性的理论，利用植物体离体的器官 (如根、茎、叶、花、茎尖、果实等)、组织 (如形成层、表皮、表层、髓部细胞、胚乳等) 或细胞 (大孢子、小孢子、体细胞等) 以及原生质体，在适宜的人工培养基及环境条件下，能够诱导出愈伤组织、不定芽、不定根、最后形成完整植株的技术。
B5 培养基特点是铵盐含量较低，硝酸盐和硫胺素含量较高，主要用于培养双子叶植物，尤其是木本植物的生长。B5 维生素溶液 (1000×) 主要由肌醇、烟酸、盐酸吡哆醇、硫胺素等组成，是一种常用的培养基组分。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤 (仅供参考)：
1、使用方法：取 1ml 本品加入 1L 的培养基溶液中，混匀即可。调节至所需 pH 值，一般为 5.8±0.2。
2、根据需要，可进行高压灭菌处理，用于植物组织培养。也可加入额外的碳源、凝胶、植物激素或生长调节剂等成分。
注意事项：
1、 本品作为相应的植物组培培养基基盐的添加剂使用。
2、要根据不同的材料、不同的物种，选择合适的培养基，最好通过实验获得。
3、试剂开封后请尽快使用，以防影响后续实验效果。
4、如需严格无菌，可再次于 121℃ 高压灭菌 15~20min。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
6、本品为实验室科研用试剂，仅供科研实验使用，不可用于人体、临床诊断、食品及家庭用途等。
Plant tissue culture, also known as axenic culture or in‑vitro culture technology, is based on the theory of plant cell totipotency. Using explants including isolated plant organs (roots, stems, leaves, flowers, shoot‑tips, fruits, etc.), tissues (cambium, epidermis, cortical‑pith cells, endosperm, etc.), cells (megaspores, microspores, somatic cells, etc.) and protoplasts, this technique enables the induction of callus, adventitious buds and adventitious roots under suitable artificial culture medium and environmental conditions, and finally regenerates intact plantlets.
B5 medium is characterized by low ammonium‑salt content and relatively high nitrate and thiamine levels. It is mainly applied to the cultivation of dicotyledons, especially woody plants. B5 Vitamin Solution (1000×) is mainly composed of myo‑inositol, nicotinic acid, pyridoxine hydrochloride, thiamine and other components, and serves as a common medium supplement. This reagent is intended for research‑only use and shall not be used for clinical diagnosis or other purposes.
1. Directions for use: Add 1 mL of this product to 1 L of medium solution and mix thoroughly. Adjust the pH to the required value, generally 5.8±0.2.
2. If necessary, the medium can be autoclaved for plant‑tissue‑culture applications. Additional carbon sources, gelling agents, plant hormones or plant‑growth‑regulators may also be supplemented.
Precautions:
1. This product is used as an additive for corresponding plant‑tissue‑culture basal salt medium.
2. Select a suitable culture medium according to different experimental materials and plant species; optimal conditions are best determined experimentally.
3. Use the reagent as soon as possible after opening to avoid impairing subsequent experimental results.
4. If strict sterility is required, autoclave again at 121 ℃ for 15‑20 min.
5. For your safety and health, wear a lab coat and disposable gloves during operation.
6. This is a laboratory research‑grade reagent. It is for research‑only purposes and must not be used for human‑related applications, clinical diagnosis, food production or household‑use purposes.
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