I2621773计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥90% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存,干燥。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
VivoTrack 680是一种水溶性NIR（近红外）荧光细胞标记剂，可以标记多种细胞类型。 由于细胞迁移对于许多重要的体内生物学功能至关重要，因此这种标记方法有可能为炎症，免疫学和干细胞研究提供有用的工具。 VivoTrack 680可以明亮，均匀地标记细胞，而无需使用苛刻的有机物（例如DMSO或乙醇）或已知会影响细胞功能的化学物质。 标记的细胞保留了出色的生存力和正常的增殖能力，并且在体外分裂细胞中保留了超过7天的明显信号。 可以标记许多不同的细胞类型以用于体内转移或植入，并且通过NIR荧光成像以非侵入性方式将荧光原代巨噬细胞追踪到炎症部位来说明体内效用。
Ex(nm)：676
Em(nm)：696
VivoTrack 680 is a water soluble NIR (near infrared) fluorescent cell labeling agent that can label a variety of cell types. As cell migration is critical for many important in vivo biological functions, this labeling approach has the potential to provide a useful tool for research in inflammation, immunology and stem cells. VivoTrack 680 can label cells brightly and uniformly without requiring harsh organics such as DMSO or ethanol, chemicals known to affect cell function. The labeled cells retain excellent viability and normal proliferative capacity, and significant signal is retained in dividing cells in vitro for more than 7 days. Many different cell types can be labeled for in vivo transfer or implantation, and in vivo utility is illustrated by non-invasively tracking fluorescent primary macrophages to sites of inflammation by NIR fluorescent imaging.
1. Track stem cells, T cells, macrophages and many other cell types longitudinally in vivo 2. Water soluble - no need for harsh organics such as DMSO or ethanol, chemicals known to affect cell function Homogeneous cell staining with no impact on cell viability. 3.Optimized for in vivo imaging instrument
1. Wash the cells of interest once with sterile PBS or serum-free medium to remove serum proteins and lipids that may interfere with cell labeling. 2. Discard the supernatant and resuspend the cells (up to 250 x 106 cells/mL) in 2.0 mL of PBS in a 50 mL sterile conical tube. 3. Dissolve VivoTrack 680 (0.2mg of dye in 1g of PEG5000) in 1.3 mL of warm sterile PBS (37 °C) and mix by vortex until completely dissolved. This will yield 2.0 mL of the labeling agent. 4. Add 2.0 mL of the cell labeling solution to 2.0 mL of cells, and mix immediately by gentle vortexing. [Note: It may be required to optimize dose depending on the cells to be labeled.] 5. Incubate the cells for 15 min at room temperature, protected from light. 6. Dilute the cells for washing by adding 15-20 mL sterile RT PBS containing 1% FBS or complete medium, depending on your ultimate use for the cells. 7. Wash the cells 3 times with RT PBS containing 1% FBS to remove excess cell labeling agent. A final resuspension with sterile PBS alone can be used to decrease the FBS levels in the cell preparation. 8. Count, culture, or transfer cells as required by the application. VivoTrack 680 is highly soluble in aqueous solution and, thus, does not need to transition from organic to aqueous solution upon addition to the cell suspension. This allows quick dispersion into the cell medium, and very uniform cell labeling is generally achieved. For laboratory use only. This product is intended for animal research only and not for use in humans.
规格
规格或纯度
≥90%
英文名称
VivoTrack 680 NIR Fluorescent Imaging Agent
储存条件
避光,-20°C储存,干燥
运输条件
超低温运输
纯度
≥90%
名称和识别符
分子类型
未知
技术文档
高级数据
化学和物理性质
敏感性
对光和湿度敏感
质检证书（CoA,COO,BSE/TSE 和分析图谱)
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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危险品化学品经营许可证（带存储）