计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥98% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存,干燥。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
VSPPLTLGQLLS is a small peptide FGFR3 inhibitor, peptide P3, inhibits FGFR3 phosphorylation. VSPPLTLGQLLS inhibits 9-cisRA-induced tracheal lymphangiogenesis and blocks lymphatic endothelial cell (LEC) proliferation, migration, and tubule formation.
Form:Solid
IC50& Target:FGFR3|K650M-FGFR3|K644E FGFR3
In Vitro:VSPPLTLGQLLS (5 μM and 10 μM; 24 h and 48 h) inhibits human primary lymphatic endothelial cell (LEC)s proliferation, migration, and tubule formation. VSPPLTLGQLLS shows effective inhibition in FGFR3 phosphorylation in LECs and also demonstrated to be effective in ATDC5 chondrogenic cells, 293T cells, explanted metatarsal bone cultures, and an in vivo mouse model of thanatophoric dysplasia II[2] . VSPPLTLGQLLS (10 μM; 6 h) inhibits tyrosine kinase activity of FGFR3 and its typical downstream molecules, extracellular signal-regulated kinase/mitogen-activated protein kinase. VSPPLTLGQLLS (0, 1, 10, and 50 μM; 24 h and 3 or 7 days, respectively) also promotes proliferation and chondrogenic differentiation of cultured ATDC5 chondrogenic cells. VSPPLTLGQLLS (10 μM; 0-60 min) inhibits the ERK/MAPK pathway in FGFR3-expressing chondrocytic cell line ATDC5. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot Analysis Cell Line: Human primary lymphatic endothelial cell (LEC)s; ATDC5 chondrogenic cells Concentration: 10 μM Incubation Time: 0, 5, 10, 30, 45, 60 min Result: Inhibited FGFR3 phosphorylation at Tyr 724 in human primary lymphatic endothelial cell (LEC)s. Inhibited the FGF2-mediated ERK/MAPK phosphorylation in FGFR3-expressing chondrocytic cell line ATDC5. Cell Proliferation AssayCell Line: Human primary lymphatic endothelial cell (LEC)s Concentration: 2.5 μM, 5 μM, and 10 μM Incubation Time: 15 min for pre-incubation and co-incubation with 1 μM 9-cisRA for 48 hr Result: Inhibited LEC proliferation. Cell Migration AssayCell Line: Human primary lymphatic endothelial cell (LEC)s Concentration: 5 μM Incubation Time: 15 min for pre-incubation and co-incubation with 1 μM 9-cisRA for 24 hr; observed at 0, 24, 48 hr Result: Inhibited LEC migration tubule formation.
In Vivo:VSPPLTLGQLLS (1 mM; intranasal dropping; onced daily for 7 d) blocks 9-cisRA-induced lymphangiogenesis in vivo, while 9-cisRA is an isoform of vitamin A involving in AIDS-related Kaposi Sarcoma. VSPPLTLGQLLS alleviates the bone growth retardation in bone rudiments from mice mimicking human thanatophoric dysplasia type II (TDII), reversed the neonatal lethality of TDII mice. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Lymphatic reporter mice, Prox1-GFP model [1] Dosage: 1 mM Administration: Intranasal dropping; once daily for 7 days; accompanied with 1 mM 9-cisRA or not Result: Significantly inhibited total lymphatic vessel length and number of sprouts compared increase induced by 9-cisRA. Animal Model: Fgfr3 Neo-K644E/+ EIIa-Cre mice (TDII mice) from Fgfr3 Neo-K644E/+ mice crossed with heterozygous EIIa-Cre mice Dosage: 10 μM Administration: Treated for 7 days Result: Suppressed FGFR3-mediated growth inhibition in cultured murine metatarsal bones. Rescued the lethal phenotype in thanatophoric dysplasia type II (TDII) mice. Rescued the abnormal growth plate and the lung phenotypes in the TDII mice.
规格
规格或纯度
≥98%
英文名称
VSPPLTLGQLLS
生化机理
VSPPLTLGQLLS 是一种小肽 FGFR3 抑制剂，肽 P3 可抑制 FGFR3 磷酸化。VSPPLTLGQLLS 可抑制 9-cisRA 诱导的气管淋巴管生成，阻止淋巴内皮细胞（LEC）的增殖、迁移和小管形成。
储存条件
避光,-20°C储存,干燥
运输条件
超低温运输
纯度
≥98%
名称和识别符
分子类型
小分子
分子量
1224.45
技术文档
高级数据
化学和物理性质
溶解性
H2O : 50 mg/mL (40.83 mM; Need ultrasonic)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品的纯度是多少？
本产品的化学纯度（含量）为 ≥98%。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于?20 °C、避光、干燥条件下保存。请与干燥剂一同密封保存，本品对湿气敏感。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 1206896-24-3，分子式为 C56H97N13O17，分子量为 1224.45 g/mol。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
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