WST‑3 is a water‑soluble tetrazolium dye. In the presence of an electron mediator, NADH reduces it to WST‑3 formazan, which exhibits an absorption maximum at 433 nm.
Water‑soluble tetrazolium salts (WST) are prepared by introducing positive‑charge groups, negative‑charge groups or hydroxyl groups onto the benzene ring of tetrazolium salts. Positively‑charged groups such as trialkylamino groups improve the water‑solubility of formazan dyes; however, large cations tend to precipitate with organic anions such as carboxylate or phosphate ions. Although hydroxyl groups can also enhance the water‑solubility of tetrazolium salts, the resulting formazan dyes show insufficient water‑solubility. WST reagents possess sulfonic acid groups, which are incorporated directly or indirectly into benzene rings to improve aqueous solubility. Aladdin also offers several newly‑developed phenylazo‑type tetrazolium salts. These can be readily reduced by NADH or other reducing agents to yield orange or purple formazan dyes. Owing to the phenylazo moiety, their color changes in response to heavy‑metal ions. Thanks to the high water‑solubility of Aladdin WST products, stock solutions with concentrations ranging from 10 mM to 100 mM can be prepared.
Mechanism of electron transfer to tetrazolium salts
规格
规格或纯度
BioReagent, 适用于荧光分析, 用于显微镜, ≥94.5%(HPLC)
稳定性与储存
Store at -20℃ long term (5 years). Store in the dark. Desiccated.
危险品化学品经营许可证（带存储）