Taq酶全封闭抗体是我公司潜心开发的一款对 Taq 酶的 5’→3’聚合酶活性与 5’→3’ 核酸外切酶活性进行严格封闭的单克隆抗体组合试剂。在Taq酶双封闭抗体 (D1547552) 基础上进行了升级，Taq酶全封闭抗体由三株抗体以优化的比例组合而成，对 Taq 酶的 5’→3’聚合酶活性与 5’→3’核酸外切酶活性，封闭效率更高，封闭稳定性更强，适用于多重引探全预混试剂。既可防止错配模板和引物二聚体引起的非特异性扩增，也可避免引物探针或其他关键物料的降解造成的非特异性信号和扩增效率的损失，从而双重保证扩增试剂的稳定性。本产品可快速激活，只需在95℃加热 30s，即可完全释放 Taq 酶活性。使用本产品封闭 Taq DNA聚合酶，能够显著提高全预混试剂稳定性，以及低模板检出率。本产品保存于含 Tris-HCl、KCl、甘油、EDTA、Tween-20 及 CA630 的缓冲体系中。
质量控制：
1. 纯度：SDS-PAGE ≥ 95%
2. 核酸内切酶残留：2μg本品和1μg λ DNA，37℃孵育 16 小时，DNA 的电泳谱带不发生变化。
3. 核酸外切酶残留：2μg本品与1μg λ-Hind III digest DNA，37℃孵育 16 小时，DNA 的电泳谱带不发生变化。
4. Nickase 残留：2μg本品和1μg pBR322，37℃孵育 16 小时，DNA 的电泳谱带不发生变化。
5. RNase 残留：2μg本品和1.6μg MS2 RNA，37℃孵育 4 小时，RNA 的电泳谱带不发生变化。
使用方法：
本产品浓度为 5mg/mL，建议 1μL 抗体封闭 10-30 U Taq DNA 聚合酶活性，室温混匀孵育 30 min (体积大于 200mL 混匀 2 小时，更大体积客户自行进行工艺调整），-20℃保存过夜后使用。
注意：封闭比例可根据需求客户适当调整。
注意事项：
1. 尽量避免反复冻融，操作时请佩戴手套。
2. 由于不同 Taq DNA 聚合酶的比活有差异，封闭比例可以适当调整，封闭效率高于 95%为宜。
Whole-Block Taq Antibody is a meticulously developed monoclonal antibody cocktail for stringent inhibition of the 5′→3′ polymerase activity and 5′→3′ exonuclease activity of Taq polymerase. It is an upgraded version of the Dual-Block Taq antibody (D1547552). Formulated with three antibodies at an optimized ratio, Taq Full-Blocking Antibody delivers higher blocking efficiency and superior blocking stability against both 5′→3′ polymerase and 5′→3′ exonuclease activities of Taq polymerase, making it suitable for multiplex primer-probe master mix reagents. It suppresses non-specific amplification caused by mismatched templates and primer dimers, and prevents degradation of primers, probes or other critical components that would otherwise generate non-specific signals and reduce amplification efficiency, thus providing dual assurance for the stability of amplification reagents. This product features rapid activation. Complete release of Taq polymerase activity is achieved by heating at 95 °C for merely 30 s. Blocking Taq DNA polymerase with this antibody can markedly enhance the stability of master mix reagents and improve the detection rate of low-abundance templates.The product is supplied in a buffer composed of Tris-HCl, KCl, glycerol, EDTA, Tween-20 and CA630.
Quality Control:
1. Purity: ≥95% by SDS‑PAGE
2. Endonuclease residual activity: No change in DNA electrophoretic band pattern when 2 μg of this product is incubated with 1 μg λ DNA at 37 °C for 16 h.
3. Exonuclease residual activity: No change in DNA electrophoretic band pattern when 2 μg of this product is incubated with 1 μg λ‑Hind III digest DNA at 37 °C for 16 h.
4. Nickase residual activity: No change in DNA electrophoretic band pattern when 2 μg of this product is incubated with 1 μg pBR322 at 37 °C for 16 h.
5. RNase residual activity: No change in RNA electrophoretic band pattern when 2 μg of this product is incubated with 1.6 μg MS2 RNA at 37 °C for 4 h.
Instructions for Use:
This product is supplied at 5 mg/mL. It is recommended that 1 μL of antibody be used to block 10–30 U of Taq DNA polymerase activity. Mix thoroughly and incubate at room temperature for 30 min (incubate for 2 h for volumes greater than 200 mL; customers shall optimize the process for larger volumes). Store overnight at −20 °C prior to use.
Note: The blocking ratio may be adjusted appropriately by customers as required.
Precautions:
1. Avoid repeated freeze‑thaw cycles; wear gloves during handling.
2. The specific activity varies between different Taq DNA polymerase preparations, so the blocking ratio can be adjusted accordingly. A blocking efficiency of >95% is preferred.
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