计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Laemmli样品缓冲液专为蛋白质样品制备而配制,可用于Laemmli SDS-PAGE系统。将蛋白质样品与2X样品缓冲液(1:1)混合,并在沸水中加热2-5分钟。2-巯基乙醇还原分子内和分子间二硫键。SDS去污剂会使蛋白质和亚基变性,并给每个负电荷一个整体,使每个负电荷根据大小分开。溴酚蓝用作在蛋白质之前延伸的染料前缘,还可以使加载过程中更容易看到样品。甘油增加了样品的密度,因此它将很好地在样品中分层。溶液包含4%SDS,20%甘油,10%2-巯基乙醇,0.004%溴苯酚蓝和0.125 M Tris H
Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well. Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.
2X Laemmli Sample Bufferis for SDS-PAGE protein sample preparation, protein sample is mixed (1:1) with the 2X sample buffer补充资料This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
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