计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥99% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,干燥。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
YKL-5-124 TFA is a potent, selective, irreversible and covalent CDK7 inhibitor with IC 50 s of 53.5 nM and 9.7 nM for CDK7 and CDK7/Mat1/CycH , respectively. YKL-5-124 TFA is >100-fold greater selective for CDK7 than CDK9 and CDK2 , and inactive against CDK12 and CDK13 . YKL-5-124 TFA induces a strong cell-cycle arrest, inhibits E2F-driven gene expression, and exhibits little effect on RNA polymerase II phosphorylation status
In Vitro
YKL-5-124 (0-2000 nM; 72 hours; HAP1 cells) treatment causes a dose-dependent increase in G1- and G2/M-phase cells and a corresponding loss of S-phase cells. YKL-5-124 (0-2000 nM; 24 hours; HAP1 WT cells) treatment inhibits CDK1 T-loop phosphorylation, and to a lesser extent CDK2 T-loop phosphorylation in a concentration-dependent fashion. Treatment of cells with YKL-5-124 as a competitor at a concentration of about 30 nM blocks pull-down of CDK7-cyclin H but has no effect on the pull-down of cyclin K-CDK12/13 in HAP1 cells. Treatment with 100 nM YKL-5-124 reduces CDK7-cyclin H binding to bioTHZ1 by >50% at 30 min. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cycle AnalysisCell Line: HAP1 cells Concentration: 0 nM, 0.2 nM, 0.7 nM, 2 nM, 6.3 nM, 20 nM, 60 nM, 200 nM, 633.3 nM, 2000 nM Incubation Time: 72 hours Result: Caused a dose-dependent increase in G1- and G2/M-phase cells and a corresponding loss of S-phase cells. Western Blot AnalysisCell Line: HAP1 WT cells Concentration: 0 nM, 125 nM, 250 nM, 500 nM, 1000 nM, 2000 nM Incubation Time: 24 hours Result: Inhibited CDK1 T-loop phosphorylation, and to a lesser extent CDK2 T-loop phosphorylation in a concentration-dependent fashion.
Form:Solid
IC50& Target:CDK7 53.5 nM (IC 50 ) CDK7/Mat1/CycH 9.7 nM (IC 50 ) CDK2 1300 nM (IC 50 ) CDK9 3020 nM (IC 50 )
规格
别名
YKL-5-124 三氟乙酸
规格或纯度
≥99%
英文名称
YKL-5-124 TFA
生化机理
YKL-5-124 TFA 是一种强效、选择性、不可逆的共价 CDK7 抑制剂，对 CDK7 和 CDK7/Mat1/CycH 的 IC 50 s 分别为 53.5 nM 和 9.7 nM。YKL-5-124 TFA 对 CDK7 的选择性是 CDK9 和 CDK2 的 100 倍以上，而对 C
储存条件
2-8°C储存,干燥
运输条件
低温运输
纯度
≥99%
名称和识别符
分子类型
小分子
Smiles
CC1(C)N(C(N[C@@H](C2=CC=CC=C2)CN(C)C)=O)CC3=C1NN=C3NC(C4=CC=C(NC(C=C)=O)C=C4)=O.FC(F)(C(O)=O)F
分子量
629.63
技术文档
高级数据
化学和物理性质
溶解性
DMSO : ≥ 100 mg/mL (158.82 mM) H2O : 50 mg/mL (79.41 mM; Need ultrasonic)
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 SSE:688179 保留所有权利。
危险品化学品经营许可证（带存储）