计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥99% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
ZX-29 is a potent and selective ALK inhibitor with an IC 50 of 2.1 nM, 1.3 nM and 3.9 nM for ALK , ALK L1196M and ALK G1202R mutations, respectively. ZX-29 is inactive against EGFR . ZX-29 induces apoptosis by inducing endoplasmic reticulum (ER) stress and overcomes cell resistance caused by an ALK mutation. ZX-29 also induces protective autophagy and has antitumor effect
In Vitro
ZX-29 (0-81 nM; 24-72 hours; NCI-H2228 cells) treatment leads to a time- and dose-dependent decrease in NCI-H2228 cell viability. ZX-29 (10 nM; 24 hours; NCI-H2228 cells) treatment causes typical signs of autophagy and the formation of autophagosomes. ZX-29 enhances the expression level of LC3 and Beclin1. ZX-29 (10 nM; 0-48 hours; NCI-H2228 cells) inhibits the proliferation of NCI-H2228 cells and arrests the cells in G1 phase. ZX-29 (10-40 nM; 24-48 hours; NCI-H2228 cells) treatment induces apoptosis of NCI-H2228 cells. ZX-29 dose-dependently upregulates the expression levels of proapoptotic protein Bax, increases the production of activated forms of caspase 3, and downregulates the expression level of antiapoptotic protein Bcl-2. ZX-29 (30-300 nM; 24 hours; NCI-H2228 cells) treatment significantly down-regulates the expression of p-ALK and its downstream signaling proteins, including p-Akt and p-STAT3, in a dose-dependent manner. ZX-29 (20 nM; 0-48 hours; NCI-H2228 cells) treatment significantly increases the mRNA level of CHOP. ZX-29 dose-dependently inhibits colony formation of NCI-H2228 cells. With an increase in ZX-29 concentration, the cell density decreased gradually, and the cells lost their normal morphology and become sharp and slender. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: NCI-H2228 cells Concentration: 0 nM, 1 nM, 3 nM, 9 nM, 10 nM, 27 nM or 81 nM Incubation Time: 24 hours, 48 hours or 72 hours Result: Led to a time- and dose-dependent decrease in NCI-H2228 cell viability. Cell Autophagy AssayCell Line: NCI-H2228 cells Concentration: 10 nM Incubation Time: 24 hours Result: Caused typical signs of autophagy and the formation of autophagosomes. Cell Cycle AnalysisCell Line: NCI-H2228 cells Concentration: 0 hour, 12 hours, 24 hours or 48 hours Incubation Time: 24 hours Result: Arrested the NCI-H2228 cells in G1 phase in a time-dependent manner. Apoptosis AnalysisCell Line: NCI-H2228 cells Concentration: 10 nM, 20 nM or 40 nM Incubation Time: 24 hours, 48 hours Result: Promoted NCI-H2228 cell apoptosis in a dose-dependent manner. Western Blot AnalysisCell Line: NCI-H2228 cells Concentration: 30 nM, 100 nM, 300 nM Incubation Time: 24 hours Result: Significantly down-regulated the expression of p-ALK and its downstream signaling proteins, including p-Akt and p-STAT3, in a dose-dependent manner. RT-PCRCell Line: NCI-H2228 cells Concentration: 20 nM Incubation Time: 0 hour, 6 hours, 12 hours, 24 hours or 48 hours Result: The mRNA level of CHOP was increased significantly.
In Vivo
ZX-29 (50 mg/kg; intragastric administration; every 2 days; for a total of 7 times; female BALB/c nude mice) treatment suppresses tumor growth in a mouse xenograft model . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Female BALB/c nude mice (4-week-old) with H2228 cells Dosage: 50 mg/kg Administration: Intragastric administration; every 2 days; for a total of 7 times Result: Showed significantly attenuated tumor growth.
Form:Solid
IC50& Target:IC50: 2.1 nM (ALK), 1.3 nM (ALK L1196M) and 3.9 nM (ALK G1202R)
规格
规格或纯度
≥99%
英文名称
ZX-29
生化机理
ZX-29 是一种强效的选择性 ALK 抑制剂，对 ALK、ALK L1196M 和 ALK G1202R 突变的 IC 50 分别为 2.1 nM、1.3 nM 和 3.9 nM。ZX-29 对表皮生长因子受体无活性。ZX-29 通过诱导内质网（ER）应激和细胞凋亡诱导细胞凋亡。
储存条件
避光,-80℃储存
运输条件
特低温运输
纯度
≥99%
名称和识别符
分子类型
小分子
分子量
518.03
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 50 mg/mL (96.52 mM; ultrasonic and warming and heat to 60°C)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品的纯度是多少？
本产品的化学纯度（含量）为 ≥99%。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于?80 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品采用干冰配冷袋冷冻运输。收货后请立即拆包，并按上述储存条件保存；处理干冰时请戴隔热手套并保持通风。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 2254805-62-2，分子式为 C23H28ClN7O3S，分子量为 518.03 g/mol。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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