碘硝基四唑紫（INT）

四唑鎓染料前体用于测量脱氢酶活性。

规格或纯度: 98%

CAS编号: 146-68-9
分子式: C₁₉H₁₃N₅O₂ICl
分子量: 505.71
Beilstein号: 4093220
EC号: MFCD00011961
MDL号: MFCD00011961
PubChem编号: 64957

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货号 (SKU)	包装规格	是否现货
I105499-250mg	250mg	现货
I105499-1g	1g	现货
I105499-5g	5g	现货
I105499-25g	25g	现货

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基本描述

别名	碘硝基氯化四氮唑蓝\|2-(对碘苯基)-3-(对硝基苯基)-5-苯基四唑氯水合物\|2-(4-碘苯基)-3-(4-硝基苯基)-5-苯基氯化四唑氯水合物\|碘硝基氯化四氮唑\|INT\|对碘硝基四唑紫
英文别名	Iodonitrotetrazolium chloride\|146-68-9\|Iodonitrotetrazolium\|2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride\|Iodonitrotetrazolium violet\|INT\|Iodonitrotetrazolium purple\|Iodonitro tetrazolium\|p-Iodonitrotetrazolium Violet\|MFCD00011961\|XY3JA5
规格或纯度	98%
英文名称	Iodonitrotetrazolium chloride
生化机理	spectrophotometric assay for succinate dehydrogenase activity: Materials: spectrophotometer cuvette with 1-cm light patch. Reaction Mixture: 100 mmol/liter triethanolamine (HCl), pH8,3 o.5 mmol/liter EDTA, 2 mmol/liter Iodonitrotetrazolium chloride, 12 g/liter Cremophor EL, 20 mmol/liter succinate, pH7.4 Reaction: mix 990 ul of reaction mixture with 10ul mitochondrial suspension color develops at 30oC record for 6 min. @500 nm.Tetrazolium dye precursor that forms a purple formazan dye on reduction. Used as an electron acceptor for the colorimetric assay of dehydrogenase activity. Also used for western blot staining and as a staining agent for soft agar colony formation assays.
应用	色度法检测各种脱氢酶的电子受体（Electron acceptor）碘硝基四唑紫已用于细胞染色。它已被用作 D-阿拉伯糖醇的酶促测定中的偶联剂。
储存温度	2-8°C储存
运输条件	冰袋运输
备注	如果有可能，您尽量在使用的当天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。
产品介绍	Iodonitrotetrazolium chloride is an electron acceptor used in various colorimetric assays. The chemical has been mainly documented for its measurement of dehydrogenase activity. In the assay to measure SDH (succinate dehydrogenase) activity, Iodonitrotetrazolium chloride was used as an oxidizing agent being reduced by succinate dehydrogenase. Upon reduction the compound forms a formazan dye measured at 500 nm. Iodonitrotetrazolium chloride has also been used to study the electron transport activity in soils, sediments, and pure enzyme cultures as well as nucleotide diphosphate kinase activity.色度法检测各种脱氢酶的电子受体（Electron acceptor）碘硝基四唑紫已用于细胞染色。它已被用作 D-阿拉伯糖醇的酶促测定中的偶联剂。 Iodonitrotetrazolium chloride is an electron acceptor used in various colorimetric assays. The chemical has been mainly documented for its measurement of dehydrogenase activity. In the assay to measure SDH (succinate dehydrogenase) activity, Iodonitrotetrazolium chloride was used as an oxidizing agent being reduced by succinate dehydrogenase. Upon reduction the compound forms a formazan dye measured at 500 nm. Iodonitrotetrazolium chloride has also been used to study the electron transport activity in soils, sediments, and pure enzyme cultures as well as nucleotide diphosphate kinase activity. An electron acceptor that forms a formazan dye on reduction; measures dehydrogenase activity.

名称和标识符

EC号	MFCD00011961
IUPAC Name	2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazol-3-ium;chloride
INCHI	InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
InChi Key	JORABGDXCIBAFL-UHFFFAOYSA-M
Canonical SMILES	C1=CC=C(C=C1)C2=NN([N+](=N2)C3=CC=C(C=C3)[N+](=O)[O-])C4=CC=C(C=C4)I.[Cl-]
Isomeric SMILES	C1=CC=C(C=C1)C2=NN([N+](=N2)C3=CC=C(C=C3)[N+](=O)[O-])C4=CC=C(C=C4)I.[Cl-]
WGK Germany	3
PubChem CID	64957
分子量	505.71
Beilstein号	4093220
Reaxy-Rn	3846329

化学和物理性质

溶解性	Soluble in water (4 mg/ml), ethanol (8 mg/ml), ethylene glycol (30 mg/ml), DMSO (slightly), and methanol: water (1:1, 50 mg/ml, very faintly turbid, very deep yellow,hot).
敏感性	易吸潮,对热及光线敏感
熔点	231°C

安全和危险性（GHS）

象形图	Harmful
信号词	Warning
危险声明	H315: Causes skin irritation H319: Causes serious eye irritation H335: May cause respiratory irritation H302: Harmful if swallowed H312: Harmful in contact with skin H332: Harmful if inhaled
预防措施声明	P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P271,P270,P304+P340,P403+P233,P362+P364,P330,P264+P265,P301+P317,P317,P337+P317,P332+P317,P319
WGK Germany	3
Reaxy-Rn	3846329

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INT在水中的溶解度多少？用于测试脱氢酶活性的0.2%INT溶液应如何配置

问题由江丽华于 2022年6月13日 22:32 创建

尊敬的客户您好，
您在我司提交的【 I105499】咨询问题,给您的回复如下：

您好，该产品不溶于水，可以溶于DMSO和乙醇，具体溶剂的选择建议您以实验方案为准。

祝您工作愉快！欢迎您再次咨询。
上海阿拉丁生化科技股份有限公司
2022年6月14日

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碘硝基四唑紫（INT）

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基本描述

名称和标识符

化学和物理性质

安全和危险性（GHS）

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