INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Irfan Ijaz,Aysha Bukhari,Ammara Nazir,Ezaz Gilani,Hina Zain,Attia Shaheen,Mohammed Rafi Shaik,Mujeeb Khan
Lowering the threat of antibiotics is a hot issue in environmental chemistry. To overcome this problem, a novel MBene-based adsorbent, DTT@BC@MoBT x , was designed by modification of bacterial cellulose (BC) using MoBT x MBene and 1,4-dithiothreitol (DTT). Several characterization methods, including Fourier transforms infrared (FTIR), X-ray photoelectron spectroscopy (XPS), and X-ray Diffraction (XRD), confirmed the successful synthesis of DTT@BC@MoBT x composite. It was discovered that the abundance of functional groups, higher specific surface area, and porosity were facilitative for DTT@BC@MoBT x adsorbents to indomethacin (IDM) and losartan (LP). The adsorption of IDM and LP well fitted to the pseudo-second-order kinetic model and Langmuir isotherm model, suggesting the single-layer chemisorption occurred on the adsorbent. The maximum sorption capabilities of the DTT@BC@MoBT x adsorbent were 1041.26 mg/g for IDM and 887.31 mg/g for LP, indicating excellent adsorption performance. The adsorption capacities of IDM and LP showed a slight decline after four consecutive adsorption-desorption tests, indicating excellent regeneration ability and stability. Interfering monoanionic (Na + and K + ) and monoanionic (Cl − and NO 3 − ) exhibited negligible impacts on IDM and LP elimination rates at all three concentrations. Additionally, it was discovered that metal ions had a greater ability for interference in a higher valency state (Mg 2+ , Ca 2+ , SO 4 2− , and CO 3 2− ) than in a lower valency state (Na + , K + , Cl − , and NO 3 − ). The adsorption processes of IDM and LP by the DTT@BC@MoBT x adsorbent involved hydrogen bonding and electrostatic attraction, as shown by FTIR and XPS analyses. This work offered a novel MBene-based adsorbent for the quick and effective adsorption of IDM and LP from water.
研究领域:
环境化学
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材料科学
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DOI
PubMed
高影响因子
CARBOHYDRATE RESEARCH
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2021
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IF 2.104
Lin Yue,Meihong Zheng,Min Wang,Imran Mahmood Khan,Bin Wang,Xiaoyuan Ma,Chifang Peng,Zhouping Wang,Wenshui Xia
The objectives of the present study are to synthesize a series of chitosan oligosaccharide-O-Terpenol (COS–O-Ter) derivatives and their implication to evaluate in vitro antibacterial activity . Herein, a general strategy is described for preparing COS–O-Ter derivatives, including substitution and deprotection reactions . The structures of COS–O-Ter derivatives were characterized by FT-IR, 1 H NMR, XRD, TGA , and elemental analysis . COS–O-Ter derivatives revealed the excellent solubility and in vitro antibacterial activity. Moreover, their antibacterial activities were more sensitive to Staphylococcus aureus ( S. aureus ) than Escherichia coli ( E. coli ) indicating the effective potential application of COS–O-Ter derivatives as natural antibacterial agents . The aforementioned study opens a pave to expand the application scope of COS and its derivatives in the food and pharmaceutical industries .
研究领域:
生物化学
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医药学
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食品科学
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DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2021
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IF 4.36
Yuanyuan Chai,Yunxia Xu,Ziyin Xia,Xin Huang,Luyong Zhang,Zhenzhou Jiang
Ethnopharmacological relevance Zhuanggu Guanjie Pill (ZGGJP), a modern Chinese medicine formula, is composed of 12 herbs and has been used to treat osteoporosis in China for almost 30 years. However, no in vivo study of the influences of ZGGJP on the cytochrome P450 (CYP) activities have been reported. Aim of the study The aim of this study was to evaluate the effects of ZGGJP on the activities and the mRNA expression of CYP enzymes (CYP1A2, CYP2B6 , CYP2C9 , CYP2C19 , CYP2D6 , CYP2E1 and CYP3A) and their corresponding nuclear receptor levels in rats. Materials and methods After 7 days oral treatment of ZGGJP at low- and high-dose, cocktail solution was given to rats. Blood samples were collected at series of time points. The plasma concentrations of probe drugs and their corresponding metabolites were determined by UPLC-MS/MS. The influence of ZGGJP on the activities of seven CYPs were evaluated the metabolic ratios (C max and AUC 0-t) for metabolites/probe drugs. In addition, the effects of ZGGJP on the mRNA expression of CYPs and their corresponding nuclear receptors in rat liver were evaluated by real-time PCR. Results ZGGJP showed significant inductive effects on CYP1A2 and CYP2B6 of both male and female rats. The influence of ZGGJP on CYP2C9 and CYP3A showed gender difference. ZGGJP could induce the activities of CYP2C9 and CYP3A in female rats, but have no influence on the activities in male rats. ZGGJP had no effects on CYP2D6, CYP2C19 and CYP2E1. The mRNA expression results of CYPs were in accordance with the pharmacokinetic results. The mRNA expression levels of constitutive androstane receptor (CAR) and vitamin D receptor (VDR) were increased significantly in female rats at high dosage, but no significant changes were observed in male rats. Conclusion ZGGJP had inductive effects on CYP1A2 and CYP2B6 in both male and female rats. The results showed that ZGGJP could induce the activities of CYP2C9 and CYP3A in female rats, but had no effect in male rats. This may suggest that the influence of ZGGJP on CYP2C9 and CYP3A exhibit gender difference. The inductive effects of ZGGJP on the activities of CYPs, exhibiting gender difference, may be regulated by CAR and VDR. Therefore, co-administration of ZGGJP with other drugs, especially using CYP2C9 and CYP3A substrates in females, may need dose adjustment to avoid herb-drug interaction.
研究领域:
民族药理学
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药物代谢
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分子生物学
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DOI
PubMed
高影响因子
CHROMATOGRAPHIA
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2022
·Vol. 85
·期 3
·221-231
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IF 2.213
Sun Zhiping,He Lingli,Yang Qingqing,Zhang Haizhi,Xu Weiren,Qin Xinguang,Liu Gang,Hu Zhongze,Zhang Luyong,Liu Changxiao
The influence of genetic polymorphism of metabolic enzymes on drug–drug interactions (DDI) should be thoroughly investigated owing to its remarkable effect on therapeutic treatments that are futile. Here, different from earlier articles focused on various CYP subfamilies and their isoforms, the effect of genetic polymorphs of a particular isoform, CYP2C9, was comprehensively studied. Using diclofenac as probe substrate, the influence of three CYP2C9 variants (CYP2C9*1, *2 and *3) on DDI was conducted from the measurement of inhibitory ability of six drugs towards three variants. A modified LC–MS/MS method according to former report was constructed for the determination of 4ʹ-hydroxydiclofenac in CYP2C9 enzyme incubation system and validated for accuracy, precision, linearity range within the acceptance criteria for regulatory guidelines. After selecting appropriate incubation time and enzyme concentration, the kinetic parameters of three CYP2C9 variants towards diclofenac were examined and half inhibitory concentrations (IC 50 ) of six drugs towards three CYP2C9 variants were determined. IC 50 of paroxetine and losartan towards CYP2C9*2 were almost threefold higher and 2.5-fold lower than that towards CYP2C9*1, respectively. IC 50 of glibenclamide towards CYP2C9*3 was twofold more than that towards CYP2C9*1. Our results could offer effective guidelines for co-administration of these six drugs with various CYP2C9 substrates in individuals carrying variant CYP2C9 alleles, also broaden the application area of LC–MS/MS.
研究领域:
药理学
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临床医学
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分子生物学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
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2022
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IF 10.723
Chang Li,Benzhi Ju,Shufen Zhang
Catalyst-free fully bio-based hydroxyester (BHE) vitrimers were synthesized by crosslinking and plasticizing epoxidized soybean oil with synthesized acetylated starch succinate monoesters to investigate the effects of different starch structures on the properties of the BHE vitrimers. The BHE vitrimers possessed a lower glass transition temperature as well as better solvent resistance and reprocessing performance compared to traditional starch-based materials. Owing to dynamically covalent bonds , the migration and exudation of plasticizers were avoided. A maximum strain of 230 % was achieved to prevent the retrogradation and brittleness of starch-based materials. Furthermore, the mechanical properties remained unchanged after three reprocessing cycles. Consequently, the obtained BHE vitrimers are eco-friendly and sustainable.
研究领域:
材料科学
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化学工程
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生物技术
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2023
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IF 4.3
Qinglin Wang,Hao Yuan,Xinyue Pan,Yiling Yang,Xiaosong Ma,Yahui Guo
The purpose of this study was to explore the feasibility and mechanism of synthesizing silver nanoclusters (AgNCs) using a double-stranded DNA (dsDNA) template and to use the AgNCs as probes for captopril detection. The results showed that the binding of silver ions towards DNA bases deduced the destruction of the initial dsDNA structure , and AgNCs with decent quantum yield (43.54%) were obtained. The detection using these AgNCs probes showed good performance for captopril , with a linear range of 0.1–0.8 μg·mL −1 and a detection limit of 0.045 μg·mL −1 . Recovery results in real samples of Apocynum venetum leaf tea and Ginkgo biloba leaf tablet demonstrated its good applicability. This work not only extended the understanding of DNA-AgNCs but also provided a fast and cost-effective tool for captopril detection.
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DOI
高影响因子
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
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2023
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IF 3.9
Shasha Liu,Ruohan Cheng,Hui He,Kunming Ding,Rongmi Zhang,Yuanyuan Chai,Qinwei Yu,Xin Huang,Luyong Zhang,Zhenzhou Jiang
This study aimed to investigate the effect and mechanism of 8-methoxypsoralen (8-MOP) on acetaminophen (APAP)-induced hepatotoxicity in mice. The study found that 1 h after intraperitoneal injection of 300 mg/kg APAP, treatment with 40 mg/kg, 80 mg/kg and 120 mg/kg 8-MOP could reduce serum transaminase level and histopathological liver necrosis area. Elevated mRNA expression of liver inflammatory mediators caused by excessive APAP was also reversed. 8-MOP significantly reduced APAP-induced hepatotoxicity dose-dependently, and the highest therapeutic dose of 8-MOP (120 mg/kg) had no harmful effects on the liver. Cocktail probe assay revealed that 8-MOP can inhibit Cyp2e1 enzymatic activities of mice, thereby reducing the production of acetaminophen-cysteine (APAP-CYS), a toxic metabolite of APAP. 8-MOP had no significant effect on the protein and gene expression of Cyp2e1. The three-dimensional structures of mouse Cyp2e1 were constructed by homologous modeling. Molecular docking showed that 8-MOP had a good binding effect on the enzyme activity site of Cyp2e1. In summary, 8-MOP dose-dependently attenuated APAP-induced hepatotoxicity by binding to Cyp2e1 and occupying the active center of the enzyme, thus competitively inhibiting the oxidative metabolism of APAP, and reducing the generation of toxic product APAP-CYS.
研究领域:
药理学
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毒理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
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