INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yuying Zhang,Na Li,Lianmei Chen,Xueru Sheng,Bing Wang,Jian Zhang,Qingwei Ping,Yan Shi
In this paper, bamboo biochar was prepared and used for laccase immobilization. Biochar was prepared under different conditions (pyrolysis temperature, heating rate and dwell time) to understand biochar characteristics impacts on enzyme activity. The results showed that the preparation conditions had an important effect on the content of carboxyl groups on biochar. And the specific surface area is not the key factor affecting laccase immobilization in this study, which is a little different than before. The highest immobilized laccase activity (1404.17 U/g) was obtained when the biochar was heated to 300 °C at the rate of 15 °C/min and stayed for 1.5 h, and the carboxy group concentration was 0.490 mmol/g. Compared with free laccase (FL), the immobilized laccase on bamboo biochar (LBC) showed higher thermo-tolerant performance, more excellent acid-proof ability and reusability. Without any mediators, LBC displayed high degradation efficiency (74.72 %, 85.88 % and 94.53 %, respectively) for bisphenol A (BPA), malachite green (MG) and methyl orange (MO) in water. Our research demonstrates that the content of carboxyl group in biochar plays a decisive role in the immobilization of laccase and LBC has excellent performance in the effective removal of toxicant in water, which makes it a promising candidate for environmental recovery.
研究领域:
环境科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·
IF 2.7
Yuying Zhang,Na Li,Lianmei Chen,Fangrui Yu,Xueru Sheng,Jian Zhang,Qingwei Ping,Hongbin Li
Laccase immobilization is an effective way to realize the industrial application of laccase, but the loss of immobilized laccase activity is serious, which is an urgent problem to be solved. In this study, a novel laccase immobilization material was prepared by mixing waste Mn–Cu-loaded activated carbon with cellulose solution and regenerated in hydrochloric acid solution, which significantly improved the immobilized laccase activity. The optimum immobilization conditions (T, pH and initial laccase concentration) of Mn–Cu-AC/cellulose beads (Mn–Cu-ACC) for laccase were studied by the response surface method. Under the optimum conditions (T = 43 °C, pH = 4.0 and c = 6.8 g L−1), immobilized laccase (Laccase@Mn–Cu-ACC) showed excellent performance in terms of pH stability, thermostability and storage stability. And the enzyme activity of the laccase immobilized on the Mn–Cu-ACC carrier remarkably increased by 29.6% compared to that of the ACC. At last, the Laccase@Mn–Cu-ACC was used for the degradation of bisphenol A (BPA). Under the optimum conditions (t = 5.5 h, T = 45 °C, pH = 4.0 and initial cBPA = 70 mg L−1), the removal rate of BPA reached 72.3% in the first cycle, and after 5 cycles of reusing the immobilized laccase, the relative removal rate of BPA was retained at 53.2% of the initial removal rate. This study developed a new type of immobilized laccase carrier and provided a new path for the application of waste heavy metal loaded activated carbon.
研究领域:
生物化学
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环境科学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
Results in Engineering
·
2022
·
IF 0
Shiqian Li,Benkun Qi,Jianquan Luo,Yinhua Wan
Magnetic Fe 3 O 4 nanoparticles were successfully functionalized with the low-cost mussel-inspired tannic acid (TA)/polyethylenimine (PEI) under mild conditions and then activated with glutaraldehyde (GA) to covalently immobilize laccase for degradation of four model phenolic inhibitors commonly found in lignocellulosic hydrolysate. The immobilized laccase showed the highest relative enzyme activity with the laccase loading of 39.9 mg/g and the enzyme activity recovery of 53.1%. Compared with free laccase, immobilized laccase possessed better relative enzyme activity at acidic pH and wide temperature range, as well as exhibited excellent storage stability and satisfactory reusability. Moreover, it was found that the phenolic degradation products by immobilized laccase did not exhibit obvious inhibition on subsequent enzymatic cellulose hydrolysis. Thus, the low-cost TA/PEI coating for modification of magnetic nanoparticles will provide a promising support for laccase immobilization and the immobilized laccase has great potential in removal of phenolic inhibitors present in lignocellulosic hydrolysate.
研究领域:
生物化学
·
环境科学
✓ 已验证
DOI
JOURNAL OF HAZARDOUS MATERIALS
·
2018
·
IF 6.434
Binbin Shao,Zhifeng Liu,Guangming Zeng,Yang Liu,Xin Yang,Chengyun Zhou,Ming Chen,Yujie Liu,Yilin Jiang,Ming Yan
In this study, the hollow mesoporous carbon spheres (HMCs) were synthesized and modified for laccase (Lac) immobilization, and the structural characteristics of HMCs materials were determined by FESEM, TEM and FTIR etc. The maximum loading of Lac on the HMCs materials could reach 835 mg/g, meanwhile, the immobilized Lac exhibited excellent thermo-stability, pH stability, storage stability and reusability. The antibiotics removal experiments indicated that the immobilized Lac possess efficient removal efficiency for both tetracycline hydrochloride (TCH) and ciprofloxacin hydrochloride (CPH) in the presence of redox mediator. The synergy of the adsorption by HMCs and the degradation by Lac could be the reasons for the high removal of antibiotics. Meanwhile, for investigating degradation mechanism, the degradation product analysis and molecular docking method had been introduced to this study. According to the degradation products, dehydroxylation and demethylation are major degradation reactions for TCH degradation, and the oxidation of the piperazinyl substituent and hydroxylation are the major degradation for CPH degradation. The docking results showed that some important residues played the key role in the degradation process. This study indicated that the immobilization of Lac on HMCs could be potentially applied in environmental remediation of antibiotics.
研究领域:
环境科学
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生物技术
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Wei Zhang,Min Zhang,Jifei Song,Yifei Zhang,Binbin Nian,Yi Hu
To fulfill the requirements of environmental protection, a magnetically recoverable immobilized laccase has been developed for water pollutant treatment. In order to accomplish this objective, we propose a polydopamine-coated magnetic graphene material that addresses the challenges associated with accumulation caused by electrostatic interactions between graphene and enzyme molecules, which can lead to protein denaturation and inactivation. To achieve this, we present a polydopamine-coated magnetic graphene material that binds to the enzyme molecule through flexible spacer arms formed by ionic liquids . The immobilized laccase exhibited a good protective effect on laccase and showed a high stability and recycling ability. Laccase-ILs-PDA-MGO has a wider pH and temperature range and retains about 80% of its initial activity even after incubation at 50 °C for 2 h, which is 2.2 times more active than free laccase. Furthermore, the laccase-ILs-PDA-MGO exhibited a remarkable removal efficiency of 97.0% and 83.9% toward 2,4-DCP and BPA within 12 h at room temperature. More importantly, laccase-ILs-PDA-MGO can be recovered from the effluent and used multiple times for organic pollutant removal, while maintaining a relative removal efficiency of 80.6% for 2,4-DCP and 81.4% for BPA after undergoing seven cycles. In this study, a strategy for laccase immobilization by utilizing ILs spacer arms to modify GO aims to provide valuable insights into the advancement of efficient enzyme immobilization techniques and the practical application of immobilized enzymes in wastewater treatment .
研究领域:
环境科学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Engineered Fibers and Fabrics
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2016
·
IF 0.538
Quan Feng,Dingsheng Wu,Shan Huan,Man Li,Xin Li
In this paper, a novel nano-material was prepared, and then was applied for enzyme immobilization. Amidoxime ployarcylonitrile /montmorillonite (AOPAN/MMT) composite nanofibrous membranes were generated by electrospinning and amidoxime modification, then the nanofiber membranes were modified by glutaraldehyde; the modified AOPAN/MMT composite nanofibers were applied in laccase immobilization by adsorption and crosslinking method. Scanning electron microscopy was used to visualize the morphology of the nanofibers, and Fourier transform infrared spectroscopy was used to provide information on the surface chemistry of original and modified nanofibers. At the same time, the optimization of immobilization conditions and the relative properties of the immobilized laccase were also studied in this thesis. According to the experimental results the optimum conditions of laccase immobilization were obtained: glutaraldehyde concentration, crosslinking time, enzyme immobilization time were determined as 5%, 10 h and 12 h, respectively. The immobilized laccase showed a better stability resistance to temperature and pH change. Comparison of storage stabilities showed that the immobilized laccase retained more than 62.6% of its initial activity when stored at 4°C for 20 days. However, the free laccase lost most of its activity under the same conditions. The immobilized laccase retained 64.5% of its initial activity after 10 repeated batches of reaction.
研究领域:
生物技术
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材料科学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Industrial Textiles
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2017
·
IF 1.75
Quan Feng,Xin Li,Dingsheng Wu,Suo Liu,Mao Ye,Shan Huan
AOPAN nanofibers were prepared by electrospinning and amidoxime modification, subsequently, HEMA was used as the monomers for surface grafting via atom transfer radical polymerization, followed by coordination with Cu(II) ions, thereafter, the nanofibers AOPAN-poly(HEMA)-Cu(II) were explored as the novel support for laccase immobilization. Scanning electron microscopy was used to visualize the morphology of the nanofibers, and Fourier transform infrared spectroscopy was used to provide information on the surface chemistry of nanofibers. At the same time, the optimization of immobilization conditions and the relative properties of the immobilized laccase were also studied in this paper. The study showed the largest amount of immobilized laccase while the reaction time of atom transfer radical polymerization was 4 h. The immobilized laccase showed a better stability resistance to temperature and pH change, and the initial activity of immobilized laccase retained (60.3 ± 3.1)%, that of the free laccase retained only (21.3 ± 2.1)% when stored at 4℃ for 24 days. Immobilized laccase maintained its initial activity after 10 repeated batches of 64.5%.
研究领域:
生物材料学
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酶工程
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Siqin He,Bin Xu,Ying Liu,Xiaoshuang Yin,Wenzhong Yang,Yun Chen
Polyaminoamide (PAMAM) dendrimers with different generations from 1 G to 5 G were grafted on magnetic core-shell Fe 3 O 4 -SiO 2 composite particles , respectively. Using Fe 3 O 4 as a magnetic center and SiO 2 as the connecting layer, the PAMAM dendrimers with amino functional groups were modified on SiO 2 successfully, while laccase was immobilized to the surface of Fe 3 O 4 -SiO 2 -PAMAM x by covalent cross-linking using amide bond. Under the optimal conditions, the loading capacity of laccase reached 65.5 mg/g, and about 62.8 % of its initial activity of free laccase was reserved. Immobilized laccase degradated 2,4-dichlorophenol (2,4-DCP) with a degradation ratio of 91.5 % after 24 h. The degradation ratio of 82.9 % was maintained after 5 cycles of the operation, and when enhanced to the 10 cycles degradation, the degradation rate was still remained close to 80 %. Compared with free laccase, the comprehensive stability of immobilized laccase was improved significantly, and it displayed the excellent catalytic degradation ability for 2,4-DCP.
研究领域:
环境科学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Fanpeng Ran,Yulin Zou,Yixuan Xu,Xiaoyan Liu,Haixia Zhang
A kind of core-shell material with regular shape and size, and uniform composition, is prepared in aqueous phase and used for laccase immobilization. Magnetic Fe 3 O 4 nanoparticle is coated with MoS 2 by self-assembly, and the Fe 3 O 4 @MoS 2 is further coated by polyethyleneimine, which acts as a spacer arm and is beneficial to enzyme immobilization. The nanocarrier affords the high loading capability of 120 mg/g and satisfied activity recovery of 90% for laccase. The immobilized laccase is stable in a wider range of pH (3–10), temperature (20–70 °C) and storage time (1–14 days), as well as having the recyclability (1–10 cycles). The carcinogenic persistent organic pollutants (malachite green, bisphenol A, bisphenol F) in the water are degraded by the immobilized laccase with a higher efficiency (82.7%, 87.6%, 70.6%) than free laccase (14.4%, 85.0%, 48.0%), respectively. It is prospected that the constructed material is qualified as green nanoreactor for biological macromolecules immobilization.
研究领域:
环境科学
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生物技术
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材料科学
✓ 已验证
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2022
·Vol. 45
·期 12
·1955-1966
·
IF 3.434
Liu Runtang,Wang Silin,Han Mengyao,Zhang Wei,Xu Huajin,Hu Yi
In this study, an amino-functionalized ionic liquid-modified magnetic chitosan (MACS-NIL) containing 2,2-diamine-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) was used as a carrier, and dialdehyde starch (DAS) was used as a cross-linking agent to covalently immobilize laccase (MACS-NIL-DAS-lac), which realized the co-immobilization of laccase and ABTS. The carrier was characterized by Fourier infrared transform spectroscopy, scanning electron microscopy, thermogravimetric analysis, X-ray diffraction analysis, electron paramagnetic resonance, etc. The immobilization efficiency and activity retention of MACS-NIL-DAS-lac could reach 76.7% and 69.8%, respectively. At the same time, its pH stability, thermal stability, and storage stability had been significantly improved. In the organic pollutant removal performance test, the removal rate of 2,4-dichlorophenol (10 mg/L) by MACS-NIL-DAS-lac (1 U) could reach 100% within 6 h, and the removal efficiency could still reach 88.6% after six catalytic runs. In addition, MACS-NIL-DAS-lac also showed excellent degradation ability for other conventional phenolic pollutants and polycyclic aromatic hydrocarbons. The research results showed that MACS-NIL-DAS fabricated by the combination inorganic material, organic biomacromolecules, ionic liquid, and electron mediator could be used as a novel carrier for laccase immobilization and the immobilized laccase showed excellent removal efficiency for organic pollutants.
研究领域:
生物技术
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环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Fangrui Yu,Na Li,Lianmei Chen,Yuying Zhang,Xueru Sheng,Bing Wang,Jian Zhang,Qingwei Ping,Hongbin Li
In this paper, an effective strategy to improve the stability and recovery convenience of immobilized laccase by preparing magnetic particles from cheap filter paper fibers was studied, that is, MC@Fe3O4 microparticles were prepared by simple cellulose regeneration and carboxyl group functionalization, and then immobilized laccase under mild conditions. The Box-Behnken design (BBD) was used to optimize the enzyme concentration, pH value and contact time affecting the immobilization yield. The results showed that the concentration of laccase and pH value had important effects on the immobilization yield. The thermal stability, pH stability and storage stability of immobilized laccase were higher than those of free enzyme. Most importantly, the immobilized laccase had a good degradation capacity for bisphenol A (BPA), with a degradation rate of 75.65 % in the first cycle, and the BPA removal rate remained above 35 % even after 8 consecutive reuses. This study developed a new magnetic cellulose material to immobilize laccase, and the immobilized laccase had great potential in BPA wastewater treatment.
研究领域:
生物化学
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环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Chengyu Zhang,Shengping You,Yudong Liu,Chengyu Wang,Qisheng Yan,Wei Qi,Rongxin Su,Zhimin He
The raw material of resin, Bisphenol A (BPA), is an endocrine-disrupting compound that can be continuously released into the environment and directly harms health. In this study, luffa sponge was used as the raw material to prepare magnetic carbon chemicals for laccase immobilization and BPA degradation. The MLC-1 was synthesized by one-step carbonization-magnetization method, which showed good magnetic properties and a strong load capacity for laccase. Compared with free laccase, Laccase@MLC-1 showed stronger thermal stability, better acid-tolerate performance and reusability. Moreover, Laccase@MLC-1 showed higher BPA degradation efficiency than free laccase. 100 mg/L of BPA can be completely removed by Laccase@MLC-1 in 4 h, while only 62.70% of BPA was removed by the same amount of free laccase. By improving reuse strategies, a complete BPA degradation ratio was obtained in each reoperation process. All results proved that Laccase@MLC-1 might be a suitable biocatalyst candidate for BPA removal.
研究领域:
环境科学
·
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）