Laser & Photonics Reviews
·
2024
·
IF 9.8
Zhi Chen,Hao Huang,Jiefeng Deng,Changle Meng,Yule Zhang,Taojian Fan,Lude Wang,Shuo Sun,Yi Liu,Huiling Lin,Shuang Li,Yunpeng Bai,Lingfeng Gao,Junle Qu,Dianyuan Fan,Xueji Zhang,Han Zhang
Genetic engineering faces persistent challenges in achieving precise Deoxyribonucleic acid (DNA) cleavage, especially with the limitations associated with current enzyme-based methods, exemplified by issues in CRISPR technologies. This study introduces a groundbreaking approach: utilizing reactive oxygen species (ROS) generated by Multiphoton Absorption (MPA)-excited Black Phosphorus Quantum Dots (BPQDs) under femto-second laser irradiation. This innovative method not only allows excitation with lower energy but also enhances overall efficiency. The integration of complementary RNA sequences facilitates high-efficiency, site-selective DNA cleavage in the system, named “TADPOLE” (Targeted DNA Precision Oriented Laser Excision). Beyond its precision in targeting arbitrary DNA sequences using quantum dots, TADPOLE harnesses the unique multiphoton absorption property of BPQDs, enabling excitation with lower-energy light sources suitable for in vivo applications in the future. Moreover, the approach integrates guiding RNA and ultrafast laser technology to provide precise control over local ROS generation and minimal heat production. This guarantees site-specific DNA cleavage while mitigating the risk of damage to non-targeted sequences. In summary, this study catalyzes advancements in enzyme-free DNA cleavage technologies, with transformative implications for genetic engineering, biotechnology, and medicine. The holistic precision, versatility, and endurance presented by TADPOLE open new avenues for targeted gene therapies and transformative applications in related fields.
研究领域:
遗传学
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生物技术
·
医学
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DOI
高影响因子
ACS Applied Bio Materials
·
2023
·
IF 4.7
Xuetong Cai,Xiaoran Zhang,Yajie Zhang,Fude Feng
Methods of DNA cleavage have broad bioapplications in gene editing, disease treatment, and biosensor design. The traditional method for DNA cleavage is mainly through oxidation or hydrolysis mediated by small molecules or transition metal complexes. However, DNA cleavage by artificial nucleases using organic polymers has been rarely reported. Methylene blue has been extensively studied in the fields of biomedicine and biosensing due to its excellent singlet oxygen yield, redox properties, and good DNA affinity. Methylene blue mainly relies on light and oxygen for DNA cleavage, and the cutting rate is slow. Here, we synthesize cationic methylene-blue-backboned polymers (MBPs) that can bind DNA efficiently and induce DNA cleavage through free radical mechanisms in the absence of light and exogenous reagents, showing high-efficiency nuclease activity. In addition, MBPs with different structures showed selectivity for DNA cleavage, and the cleavage efficiency of the flexible structure was significantly higher than that of the rigid structure. Studies on the DNA cleavage mechanism have shown that the cleavage mechanism of MBPs is not through the common ROS-mediated oxidative cleavage pathway, but through the radical of MBP• inducing DNA cleavage. Meanwhile, MBPs can simulate topoisomerase I (Topo I)-mediated topological rearrangement of superhelical DNA. This work paved a way for the application of MBPs in the field of artificial nucleases.
研究领域:
生物化学
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分子生物学
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基因编辑
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疾病治疗
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生物传感
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Xinglu Jiang,Yiwu. Qin,Jun Zhou,Wenjin Chen,Beibei Xie,Hailun Zhou,Kangjing Li,Chunyun Liang,Cong Liu,Ao Long,Shufang Zhuo,Yigeng Liu,Fengyuan Zhou
As exposed to heavy metals, they can bind to biomacromolecules and damage these molecular structures, thereby seriously threatening human's health，for example. Hence, to study the mechanism of interaction between heavy metals and biomacromolecules plays an important role in protecting human health from heavy metal hazards. Here, ssDNA and E. coli lysates were severed as biomacromolecules to analyze their molecular structure changes as exposed to gold (Au) ion and other heavy metal ions, such as Pb 2 + , Cd 2+ , Cr 2+ and Hg 2+ . Gold nanoparticles coated by E. coli lysates (ECL-Au NPs) have been produced in the process of reducing the toxicity of Au(III) by E. coli lysates. As simultaneously exposed to heavy metal ions and sunlight, Au(III) ion can be generated from ECL-Au NPs to rapidly cleave DNA through cycling between Au(0) and Au(III). Meanwhile, the electron transfers also occur in the biomacromolecules from E. coli lysates, and it must cause their structure destruction as well. In conclusion, as exposed to multiple heavy metals, sunlight can enhance the synergetic damage of biomacromolecules by heavy metals. This study provides not only a new tool for DNA cleavage, but also a new mechanism of DNA and proteins damage caused by heavy metal pollution.
研究领域:
环境科学
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分子生物学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 22
·18102-18108
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IF 3.201
Yihan Ma,Xiaoyan Zhang,Yinjia Cheng,Xiaosui Chen,Yong Li,Aiqing Zhang
Designing and constructing favorable water-dispersible fullerenes and their derivatives are of huge importance for biological applications addressing DNA-cleavage and photodynamic therapy (PDT). In the present work, a mild, green and facile synthetic approach for the preparation of C60 nanoparticles was developed for the first time via the combination of mussel-inspired chemistry and the Michael addition reaction. The resultant C60–PDA–PEI nanoparticles were characterized by transmission electron microscopy (TEM), dynamic laser scattering (DLS), Fourier-transform infrared spectroscopy (FT-IR), Raman spectra, X-ray photoelectron spectra (XPS) and thermogravimetric analysis (TGA), demonstrating that the above two-step strategy allows easy access to the preparation of highly water-dispersible fullerene derivatives. Benefiting from their unique nanostructure, the versatile C60–PDA–PEI nanoparticles display a uniform hydrodynamic size of 160 nm in water and efficient 1O2 generation under irradiation. Furthermore, the good ability of cleaving DNA under visible light at a mass concentration of 62.5 ng μL−1 gives them high potential as PDT agents. The universal approach described in this work is capable of introducing many other functional molecules onto PDA-modified fullerenes, thus extending the possible applications of fullerene-based species in many fields of biotechnology and pharmaceutical chemistry.
研究领域:
生物技术
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药学
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纳米医学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Haimeng Pei,Yiqun Zheng,Rongmei Kong,Lian Xia,Fengli Qu
We describe a highly sensitive nanoparticle–based fluorescence resonance energy transfer (FRET) probe developed without using molecular fluorophores as donors and acceptors. The success of this work relies on the strategy that DNA scission was designed to occur to the probe when target presented, which enabled the fluorescence signal “turn-on” of graphene quantum dots (GQDs) and thus quantitative analysis . In particular, amino-modified SiO 2 NPs were initially coated by GQDs to form highly emitting SiO 2 /GQDs, followed by conjunction with DNA functionalized gold nanoparticles (Au NPs–DNA) to form SiO 2 /GQDs–DNA–Au NPs composite. Owing to the FRET interactions between the GQDs and Au NPs, the fluorescence of GQDs was effectively quenched by Au NPs. When bleomycin (BLM), a model analyte , was mixed with the probe, the fluorescence signal of GQDs would be restored due to the removal of Au NPs from the SiO 2 /GQDs surface by DNA scission treatment with BLM in the presence of Fe (II). The current FRET probe shows a good linear relationship between the fluorescence intensity and the concentration of BLM in the range from 0.5 nM to 1 μM with a detection limit of 0.2 nM. The probe also shows satisfactory results for the analysis of clinical serum samples. This method provides versatility to the application of GQDs in FRET biosensing and could be potentially extended to other similar systems by replacing the linker between the GQDs and Au NPs.
研究领域:
生物化学
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纳米医学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
·
2019
·
IF 3.224
Han Manman,Cai Weilan,Li Zhimin,Peng Liang,He Lixia,Chen Min
Quercetin is a flavonoid compound widely found in vegetables, fruits, and medicinal plants . It has carbonyl and phenolic hydroxyl groups in its structure that can easily form complexes with metal ions . In this study, we reacted quercetin in water at 95 °C with five metal trace elements commonly found in plants, namely calcium, magnesium, zinc, iron(III), and copper. Each supernatant was then examined by electrospray ionization-time-of-flight mass spectrometry (ESI-TOF MS). The results showed that quercetin can form complexes with Ca 2+ , Mg 2+ , Zn 2+ , and Cu 2+ in ratios of 2:1, 3:1, and 3:2. Interestingly, after reaction with Fe 3+ in water at 95 °C, not only can quercetin‑iron(III) complexes be formed in ratios of 2:1 and 3:1, but small amounts of quercetin‑iron(II) complexes can also be formed in the same ratios. Furthermore, DNA cleavage experiments showed that when acting alone, quercetin and Cu 2+ have weak or no cleavage effects on DNA, but the complex formed after reaction in hot water cleaves DNA in a time- and concentration-dependent manner. These results indicate that complexes may form between quercetin and a variety of metal trace elements in a water decoction of plants, and that these metal complexes may be the material basis for the health-promoting and therapeutic effects of edible or medicinal plants.
研究领域:
分析化学
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生物化学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
TRANSITION METAL CHEMISTRY
·
2014
·Vol. 39
·期 5
·577-584
·
IF 1.402
Li Xin,Bi Cai-Feng,Fan Yu-Hua,Zhang Xia,Wei Xiao-Dan,Meng Xiang-Min
A ternary coordination complex 2Cu(C 14 H 8 NO 3 Cl)(C 12 H 8 N 2 )·3CH 3 OH of a Schiff base (C 14 H 8 NO 3 Cl: 4-chloroanthranilic acid–salicylaldehyde) was synthesized from 4-chloroanthranilic acid, salicylaldehyde, copper acetate and 1,10-phenanthroline and characterized by physicochemical and spectroscopic methods. X-ray crystallography shows that the copper atom is five coordinated by the imine nitrogen, two nitrogen atoms from 1,10-phenanthroline, the hydroxyl oxygen and the carboxylate oxygen atom. The interaction of the complex with calf-thymus DNA was investigated by UV absorption spectroscopy, fluorescence emission spectroscopy and viscosity. In addition, the cleavage reaction with pBR322 supercoiled plasmid DNA was investigated. The complex binds to DNA and also exhibits significant DNA cleavage activity.
研究领域:
生物无机化学
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分子生物学
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药物化学
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化学生物学
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核酸化学
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DOI
高影响因子
Scientific Reports
·
2013
·Vol. 3
·期 1
·1-8
·
IF 2.927
Wang Chong,Wu Congyu,Zhou Xuejiao,Han Ting,Xin Xiaozhen,Wu Jiaying,Zhang Jingyan,Guo Shouwu
Graphene quantum dots (GQDs) maintain the intrinsic layered structural motif of graphene but with smaller lateral size and abundant periphery carboxylic groups and are more compatible with biological system, thus are promising nanomaterials for therapeutic applications. Here we show that GQDs have a superb ability in drug delivery and anti-cancer activity boost without any pre-modification due to their unique structural properties. They could efficiently deliver doxorubicin (DOX) to the nucleus through DOX/GQD conjugates, because the conjugates assume different cellular and nuclear internalization pathways comparing to free DOX. Also, the conjugates could enhance DNA cleavage activity of DOX markedly. This enhancement combining with efficient nuclear delivery improved cytotoxicity of DOX dramatically. Furthermore, the DOX/GQD conjugates could also increase the nuclear uptake and cytotoxicity of DOX to drug-resistant cancer cells indicating that the conjugates may be capable to increase chemotherapy efficacy of anti-cancer drugs that are suboptimal due to the drug resistance.
研究领域:
纳米技术
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药理学
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肿瘤学
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2013
·Vol. 61
·期 3
·341-344
·
IF 0.879
Dong An,Zhiwen Ye
According to active groups synergetic catalysis principle, a novel phosphodiester receptor 1-( N -guanidinoethyl)-4-( N -hydroxyethyl)-piperazidine hydrochloride was firstly synthesized and the preliminary studies of its DNA cleavage activity. It is characterized and confirmed by methods as 1 H NMR and 13C NMR. A “couple hardness with softness” piperazidine is designed to connect guanidinium group and hydroxyl group; The reserch was showed that the best cleaving conditions was 7.2 in pH; Cleaving DNA by the compound reaction was oxidation process that were proved through the free radicals quenches experiments; The compound can cleaved the pUC 19 DNA by phospholipid transferance was proved by BDNPP.
研究领域:
化学
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生物化学
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分子生物学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 11
·2367-2376
·
IF 7
Lizhi Jiao,Qisi Li,Chenming Li,Jinhui Gu,Xinping Liu,Shuijian He,Zhijun Zhang
Efficient and spatiotemporally controllable cleavage of deoxyribonucleic acid (DNA) is of great significance for both disease treatment (e.g. tumour, bacterial infection, etc) and molecular biology applications (e.g. gene editing). The recently developed light-induced cleavage strategy based on catalytic nanoparticles has been regarded as a promising strategy for DNA controllable cleavage. Although the regulation based on orthogonal light in biomedical applications holds more significant advantages than that based on single light, nanoparticle-mediated DNA cleavage based on orthogonal light has yet to be reported. In this article, for the first time, we demonstrated an orthogonal light-regulated nanosystem for efficient and spatiotemporal DNA cleavage. In this strategy, tungsten oxide (WO3) nanoparticles with photochromic properties were used as nano-antennae to convert the photoenergy from the orthogonal visible light (405 nm) and near-infrared light (808 nm) into chemical energy for DNA cleavage. We verified that only the orthogonal light can trigger high cleavage efficiency on different types of DNA. Moreover, such an orthogonal light-response nano-system can not only induce significant apoptosis of tumour cells, but also effectively eliminate bacterial biofilms.
研究领域:
纳米技术
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生物医学工程
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分子生物学
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癌症治疗
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产品关联
DOI
PubMed
高影响因子
Cell Reports
·
2023
·
IF 8.8
Ting Zeng,Jie Yin,Ziwen Liu,Zhaoxing Li,Yu Zhang,Yang Lv,Mei-Ling Lu,Min Luo,Meirong Chen,Yibei Xiao
Summary The type V-K CRISPR-associated transposons (CASTs) allow RNA-guided DNA integration and have great potential as a programmable site-specific gene insertion tool. Although all core components have been independently characterized structurally, the mechanism of how the transposase TnsB associates with AAA+ ATPase TnsC and catalyzes donor DNA cleavage and integration remains ambiguous. In this study, we demonstrate that TniQ-dCas9 fusion can direct site-specific transposition by TnsB/TnsC in ShCAST. TnsB is a 3′-5′ exonuclease that specifically cleaves donor DNA at the end of the terminal repeats and integrates the left end prior to the right end. The nucleotide preference and the cleavage site of TnsB are markedly different from those of the well-documented MuA. We also find that TnsB/TnsC association is enhanced in a half-integration state. Overall, our results provide valuable insights into the mechanism and application expansion of CRISPR-mediated site-specific transposition by TnsB/TnsC.
研究领域:
分子生物学
·
基因工程
·
CRISPR技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2023
·
IF 3.8
Yong-Po Zhang,Qing He,Xin-Hui Zhou,Guang-Huan Liu,Ai-Qin Yue,Chun-Yan Gao,Jin-Zhong Zhao,Wei-Jun Du,Shi-Ping Yan
Two new Cu(І) complexes, [Cu 2 (L 1 ) 2 (μ-Br) 2 ] (1) and [Cu 2 (L 1 ) 2 (μ-Cl) 2 ] (2) were synthesized from Cu(II) halogen salts and the reduced Schiff base ligand. Stable Cu(І) complexes were obtained via in situ reduction of Cu(II) to Cu(I) and the C N was oxidize to C = N in the ligand. The structures of two complexes were characterized by X-ray single crystal diffraction analysis as well as IR and UV–Vis spectra. The two complexes have potential antioxidant capacity and exhibit efficiently self-activated DNA cleavage activity and hydroxyl radical ROS cleavage mechanism. MTT was used to assay cytotoxicity of the complexes toward a series of tumor cell and HUVEC cell lines. Both complexes exhibited higher selective cytotoxicity against tumor cells than normal cells, which is significantly superior to the positive control. The complexes could induce cell apoptosis via mitochondrial pathway by increase of ROS levels and decrease of mitochondrial membrane potential (Δψ m ). Furthermore, the complexes can obviously inhibit the migration and invasion of the quiz tumor cells, as well as inhibit the angiogenesis of HUVEC cells.
研究领域:
化学
·
生物化学
·
分子生物学
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癌症研究
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药学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）