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ActiBioPure™,天然,无动物源 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本品从非转基因大豆中提取的天然活性蛋白, 包含Bowman-Birk型大豆胰蛋白酶抑制剂 (Bowman-Birk Inhibitor,简称 BBI)和 Kunitz型胰蛋白酶抑制剂(Kunitz Trypsin Inhibitor,简称 KTI)两种类型的胰蛋白酶抑制剂。 BBI 是一种分子量约为 8KD 道尔顿的蛋白质,该蛋白质具有抑制胰蛋白酶和糜蛋白酶的特性;KTI 是一种分子量约为 23KD 道尔顿的蛋白质,该蛋白质具有抑制胰蛋白酶的特性,是一种稳定的球蛋白类型的蛋白质。 胰蛋白酶-胰凝乳蛋白酶抑制剂由 BBI 和 KTI 这两种抑制剂蛋白按一定的比例混合喷雾干燥制成。
产品特性
| 项目 | 可接受标准 |
|---|---|
| 性状 | 白色或类白色粉末 |
| 澄清度与颜色 | 澄清、无色至淡的黄色溶液 |
| 水分 | 超过 10% |
| 蛋白质含量 | 不低于 90%(以干品计) |
| 糜蛋白酶抑制剂活性 | 不低于 0.6mg 糜蛋白酶 /mg (糜蛋白酶活性不低于 40BTEE 单元 /mg) |
| 胰蛋白酶抑制剂活性 | 不低于 0.7mg 胰蛋白酶 /mg (胰蛋白酶活性约为 10000BAEE 单元 /mg) |
| 微生物限度 | 需氧菌总数小于 1000CFU/g,霉菌和酵母菌总数小于 100CFU/g |
产品优势
本品从非转基因大豆中提取,纯度高,不含其它杂蛋白酶,全工艺过程无动物源性安全风险。
产品用途
胰蛋白酶-胰凝乳蛋白酶抑制剂具有胰蛋白酶抑制剂和糜蛋白酶抑制剂活性,常用于无血清细胞培养、干细胞培养、药用辅料或其它需要抑制蛋白酶的生化实验。
This product is a natural active protein extracted from non-genetically modified soybeans, containing two types of trypsin inhibitors: Bowman-Birk Inhibitor (BBI) and Kunitz Trypsin Inhibitor (KTI). BBI is a protein with a molecular weight of approximately 8KD Daltons, which has the property of inhibiting trypsin and chymotrypsin. KTI is a protein with a molecular weight of approximately 23KD Daltons, which has the property of inhibiting trypsin and is a stable globulin-type protein. Trypsin-chymotrypsin inhibitor is prepared by spray-drying a mixture of these two inhibitor proteins, BBI and KTI, in a certain proportion.
Product Characteristics
| Item | Acceptance Criteria |
|---|---|
| Appearance | White or off-white powder |
| Clarity and Color | Clear, colorless to pale yellow solution |
| Moisture | Exceeding 10% |
| Protein Content | Not less than 90% (calculated on dry basis) |
| Chymotrypsin Inhibitor Activity | Not less than 0.6mg chymotrypsin/mg (chymotrypsin activity is not less than 40 BTEE units/mg) |
| Trypsin Inhibitor Activity | Not less than 0.7mg trypsin/mg (trypsin activity is about 10000 BAEE units/mg) |
| Microbial Limits | Total aerobic microbial count less than 1000 CFU/g, Total mold and yeast count less than 100 CFU/g |
Product Advantages
This product is extracted from non-genetically modified soybeans, with high purity, no other miscellaneous proteases, and no animal-derived safety risks throughout the entire process.
Product Uses
Trypsin-chymotrypsin inhibitor has trypsin inhibitor and chymotrypsin inhibitor activities, and is commonly used in serum-free cell culture, stem cell culture, pharmaceutical excipients, or other biochemical experiments that require protease inhibition.
Product Characteristics
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Animal Free 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 Native 级别指南 →