AIE 线粒体红色探针是一种基于AIE原理开发的四苯基乙烯衍生物，具有典型的AIE特性。产品具有细胞跨膜能力，只需简单地和细胞孵育，即可通过被动转运完成跨膜运输。
产品特性
AIE 线粒体红色探针是一种有带正电荷吡啶盐结构的离子型化合物，具有良好的聚集诱导发光特性，可以特异性标记多种细胞的线粒体结构，在与线粒体结合前后由于聚集状态的变化，其荧光强度会产生极为明显的变化，而未与线粒体结合的荧光探针基本不会发射出荧光信号，因此本产品在与细胞共同孵育后无需进行进一步的清洗便可以直接成像。
图 1. HeLa细胞上的激光共聚焦成像效果图
与常见染料不同，AIE 线粒体红色探针可通过包括488、543等在内的多个激发波长激发，并且斯托克斯位移大，可以明显与其他染料进行区分，减少成像中串色的可能性。同时AIE 线粒体红色探针的生物相容性好，成像浓度高，可在多次扫描的状态仍然保证稳定的荧光信号输出，非常适合多次成像
产品性质
化学式（Formula）
| C₄₀H₄₈F₆N₆P
|
分子量（Molecular Weight）
| 751 g/mol
|
纯度（Purity）
| ≥98% (HPLC)
|
工作浓度（Working Concentration）
| 1-20 µM
|
半峰宽（Full width at half maximum）
| 600 nm-725 nm
|
最大吸收/发射波长（nm）
| λabs=442 nm / Em= 654 nm
|
产品优势
1、细胞毒性相对较小，可以进行活细胞成像；
2、强抗光漂白能力，40 次共 15 分钟的激光扫描，发射荧光强度不变；
3、背景信号低，可免洗，快速成像。
实验方法
1、染料储备液准备：短暂离心后，向一管AIE 线粒体红色探针溶液内加入400 µL高质量的无水DMSO，吹打均匀后，配置成浓度为10 mM的AIE 线粒体红色探针储备液，适当分装后避光保存于-20 ℃或更低温度；
2、染料工作液准备：取1 µL AIE 线粒体红色探针储备液加入到1-2 mL 细胞培养液或适当的缓冲液中(例如PBS)，得到终浓度5-10 μM 的AIE 线粒体红色探针工作液，可根据实验需求调整终浓度；
3、细胞染色。适量的工作染液孵育贴壁细胞15-30 min（最好置于细胞培养箱中），免洗涤，用共聚焦荧光显微镜或荧光显微镜观察，激发波长设为488 nm或543 nm，收集550-700 nm的信号。
注意事项
1、使用前请先短暂离心；
2、第一次使用时请先配置好母液后分装，于-20℃保存，避免反复冻融；
3、为了您的安全和健康，请穿实验服并戴一次性手套操作；
4、本品仅适用于科研用途。
AIE Mitochondrion Red Probe is a tetraphenylethylene derivative developed based on the AIE principle, with typical AIE characteristics. The product has cell transmembrane ability; simply by incubating with cells, transmembrane transport can be completed through passive transport.
Product Characteristics
AIE Mitochondrion Red Probe has excellent aggregation-induced emission properties. It can specifically label lipid droplet structures in various living cells and fixed cells by targeting the polar environment of lipid droplets. Due to the change in aggregation state before and after binding to lipid droplets, its fluorescence intensity will produce extremely obvious changes, while the fluorescent probes that do not bind to lipid droplets basically do not emit fluorescent signals.
Figure 1. Laser confocal imaging effect diagram of HeLa cells
Different from common dyes, AIE Mitochondrion Red Probe can be excited by multiple excitation wavelengths including 488 nm and 543 nm, and has a large Stokes shift, which can be clearly distinguished from other dyes, reducing the possibility of crosstalk in imaging. At the same time, AIE Mitochondrion Red Probe has good biocompatibility and high imaging concentration, and can ensure stable fluorescence signal output even in the state of multiple scans, making it very suitable for multiple imaging.
Product Properties
| Product Properties | Details |
|---|
| Formula | C₄₀H₄₈F₆N₆P
|
| Molecular Weight | 751 g/mol
|
| Purity | ≥98% (HPLC) |
| Working Concentration | 1-20 µM
|
| Full Width at Half Maximum | 600 nm-725 nm
|
| Max Absorption/Emission Wavelength (nm) | λabs=442 nm / Em= 654 nm |
Product Advantages
1.Relatively low cytotoxicity, suitable for live cell imaging;
2.Strong anti-photobleaching ability; the fluorescence intensity remains unchanged after 40 laser scans totaling 15 minutes;
3.Low background signal, no need for washing, enabling rapid imaging.
Experimental Methods
1.Preparation of dye stock solution: After a brief centrifugation, add 400 µL of high-quality anhydrous DMSO to a tube of AIE Mitochondrion Red Probe solution, pipette to mix evenly, and prepare a 10 mM AIE Mitochondrion Red Probe stock solution. Aliquot appropriately and store protected from light at -20 ℃ or lower;
2.Preparation of dye working solution: Take 1 µL of AIE Mitochondrion Red Probe stock solution and add it to 1-2 mL of cell culture medium or an appropriate buffer (e.g., PBS) to obtain an AIE Mitochondrion Red Probe working solution with a final concentration of 5-10 μM. The final concentration can be adjusted according to experimental needs;
3.Cell staining: Incubate adherent cells with an appropriate amount of the working dye solution for 15-30 minutes (preferably in a cell incubator). No washing is required. Observe using a confocal fluorescence microscope or a fluorescence microscope, set the excitation wavelength to 488 nm or 543 nm, and collect signals at 550-700 nm.
Notes
1.Please perform a brief centrifugation before use;
2.When using for the first time, prepare the stock solution first, aliquot it, and store at -20℃ to avoid repeated freezing and thawing;
3.For your safety and health, please wear a lab coat and disposable gloves during operation;
4.This product is only suitable for scientific research purposes.
ZJ25F1028785
危险品化学品经营许可证（带存储）