计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
分化作用是指组织染色后用某些特定的溶液将组织过多结合的染色剂脱去,这个过程称为分化作用,所用的溶液称为分化液。在HE染色和其他染色中常用1%盐酸乙醇作为分化液,因酸能破坏苏木素的醌型结构,使组织与色素分离而退色,再进行伊红染色,才能保证细胞核与细胞浆染色的分明。
酸性乙醇分化液 (0.5%)主要由稀酸、乙醇等组成,经常用于HE染色、Masson三色染色中,更适用于要求分化力轻微的组织,是一种非常重要的辅助试剂。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、根据实验具体要求操作。
2、一般分化2-5s,立即用水或蓝化液终止。
注意事项:
1、密闭保存,一旦开启尽快用完,因为其有效成分易挥发。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
3、试剂开封后请尽快使用,以防影响后续实验效果。
Differentiation refers to the process of removing excess stain that has bound to tissues using specific solutions after tissue staining; the solution employed for this purpose is called a differentiation solution. In HE staining (Hematoxylin-Eosin staining) and other staining procedures, 1% hydrochloric acid-ethanol is commonly used as a differentiation solution. This is because the acid can disrupt the quinone structure of hematoxylin, causing the separation of tissues and pigments (thereby resulting in decolorization). Only after this step is eosin staining carried out can a clear distinction between the staining of cell nuclei and cytoplasm be guaranteed.
The Acidic Ethanol Differentiation Solution (0.5%) is mainly composed of dilute acid, ethanol, etc. It is frequently used in HE staining and Masson trichrome staining, and is more suitable for tissues that require mild differentiation power. It is an extremely important auxiliary reagent. This reagent is for scientific research use only and is not applicable for clinical diagnosis or other purposes.
Operating Procedures (for reference only)
1. Operate in accordance with the specific requirements of the experiment.
2. Generally, the differentiation process takes 2-5 seconds, and should be immediately terminated by rinsing with water or using a bluing solution.
Precautions
1. Store in a sealed container. Once opened, use it as soon as possible, as its active ingredients are volatile.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
3. After opening the reagent, use it promptly to avoid affecting the results of subsequent experiments.
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