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黑色素脂褐素染色液 (Schmorl法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
脂褐素是具有颗粒状的褐黄色色素，由含有脂肪的残存物和溶酶体消化物组成，被认为是由脂质和脂蛋白氧化产生的，氧化过程是缓慢的、逐步发生的，因此色素会呈现出不同的染色反应、不同的颜色，形状和大小也变化不一，可见于肝脏、肾脏、心肌、肾上腺、神经细胞与神经节细胞等，多分布在细胞核周围。脂褐素又可分为早期脂褐素和晚期脂褐素，早期脂褐素有脂质的所有特征，用苏丹黑B法可染成灰黑色；晚期脂褐素是经完全氧化后的色素，失去了嗜苏丹性，但有更大的还原力，主要用Schmorl高铁-铁氰化物还原法来显示；目前常用的脂褐素染色法有PAS法、Schmorl高铁-铁氰化物还原法、Gomori醛复红法等。黑色素属于非血源性内生色素，是一组颜色从浅棕色到黑色的色素，这种色素通常出现在皮肤、眼睛、大脑的黑质和毛囊中，是一种十分稳定的物质，既不溶于有机溶剂，也不溶于水；可摄取亚铁离子形成黑色素亚铁复合物，再与铁氰化钾作用成蓝色；可把氨银溶液还原为金属银；可被高锰酸钾等强氧化剂漂白。许多方法可用于识别黑色素和黑色素生成细胞，其中最可靠的有：还原方法，如Masson-Fontana银技术和Schmorl高铁-铁氰化物还原法；酶方法(如多巴反应)；荧光方法；免疫组织化学。
黑色素脂褐素染色液 (Schmorl法)的染色原理是黑色素和脂褐素均有强大的还原性，可将高铁离子还原为亚铁离子，再与铁氰化钾反应而呈暗蓝色，本法可显示任何能还原高铁化物的物质，因此对黑色素和脂褐素的显示不是特异性的，可根据组织和细胞的不同，色素的位置和分布情况以及着色深浅，对鉴别可有一定的帮助，亦可与氨银法、醛品红法、高碘酸无色品红法等进行比较观察。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
| A1508695 | Component | 2×50 mL | Storage |
| A1508695A | 高铁盐溶液 | 45 mL | RT. |
| A1508695B | 铁氰化钾溶液 | 5 mL | 2-8℃. Store in the dark. |
| A1508695C | 核复染液 | 50 mL | RT. Store in the dark. |
临用前，取A、B按9: 1混合即成Schmorl Stain，不宜提前配制。
自备材料：
固定液（10%中性福尔马林、10%福尔马林等）、系列乙醇、1%乙酸水溶液
操作步骤(仅供参考)：
(一)石蜡切片染色
1、组织固定于10%中性福尔马林，常规脱水包埋。
2、切片厚度4μm，常规二甲苯或脱蜡透明液脱蜡至水，蒸馏水水洗1min。
3、切片入Schmorl stain (见注意事项3)浸染1-5min，蒸馏水冲洗2min。
4、(可选)入1%乙酸水溶液中浸洗1-3min，充分去除铁氰化物残留，蒸馏水冲洗2min。
5、入核复染液染色3-10min，蒸馏水冲洗1-2min。
6、常规脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
(二)冰冻切片染色
1、无需脱蜡，直接迅速用蒸馏水冲洗2-3min。
2、染色、封固步骤同石蜡切片的染色步骤，时间可以相应缩短。
(三)细胞染色
1、4%多聚甲醛固定10-20min。
2、自来水冲洗2次，每次2min。
3、蒸馏水冲洗2次，每次2min。
4、染色、封固步骤同石蜡切片的染色步骤，但操作时间应相应延长。
染色结果：
脂褐素、黑色素: 绿蓝色至暗蓝色
细胞核、其他组织: 红色
注意事项：
1、切片脱蜡应尽量干净。
2、整个操作过程中尽量使用蒸馏水或去离子水，避免水中还原性杂质影响染色。
3、Schmorl stain应现用现配，一般2小时内有效；切片在Schmorl Stain中的染色时间不宜太长，一般情况下黑色素比脂褐素先着色，2min内应有反应；如染色时间太长，背景将着染很深影响对比观察。
4、1%乙酸水溶液为备选方法，可选做，乙酸浸洗可有助于去除铁氰化物残留。
5、冰冻切片和细胞染色，最好根据具体情况摸索实验条件。
6、本法可显示任何能还原高铁化物的物质，因此对脂褐素的显示不是特异性的；可根据组织和细胞的不同，色素的位置和分布情况以及着色深浅，对鉴别脂褐素有一定帮助；可与氨银法、醛品红法、高碘酸无色品红法等进行比较观察。
7、一般组织都有一定的还原性，易使背景着色，因此染色时应控制在短而恰当的时间内，达到脂褐素清晰而背景浅淡为佳。
8、为了您的安全和健康，请穿实验服并戴一次性手套操作。
9、试剂开封后请尽快使用，以防影响后续实验效果。
Lipofuscin is a granular, yellowish-brown pigment composed of lipid-containing residues and lysosomal digestates. It is believed to be generated by the oxidation of lipids and lipoproteins-a slow, progressive process that results in the pigment exhibiting variable staining reactions, colors, shapes, and sizes. Lipofuscin is found in the liver, kidneys, myocardium, adrenal glands, neurons, and ganglion cells, and is mostly distributed around the cell nucleus. It can be categorized into early-stage lipofuscin and late-stage lipofuscin. Early-stage lipofuscin retains all lipid characteristics and stains grayish-black with the Sudan Black B method. Late-stage lipofuscin is a fully oxidized pigment that loses sudanophilia but possesses stronger reducing power, and is primarily visualized using the Schmorl ferric-ferricyanide reduction method. Currently, common staining methods for lipofuscin include the PAS method, Schmorl ferric-ferricyanide reduction method, and Gomori aldehyde-fuchsin method.
Melanin is a group of non-hematogenous endogenous pigments ranging in color from light brown to black. It typically occurs in the skin, eyes, substantia nigra of the brain, and hair follicles. Melanin is a highly stable substance that is insoluble in both organic solvents and water. It can chelate ferrous ions to form melanin-ferrous complexes, which react with potassium ferricyanide to produce a blue color. Melanin can reduce ammoniacal silver solutions to metallic silver and can be bleached by strong oxidizing agents such as potassium permanganate. Numerous methods are available for identifying melanin and melanocytes, with the most reliable being: reduction methods (e.g., Masson-Fontana silver technique and Schmorl ferric-ferricyanide reduction method); enzymatic methods (e.g., dopa reaction); fluorescence methods; and immunohistochemistry.
The staining principle of Melanin-Lipofuscin Staining Solution (Schmorl Method) is Both melanin and lipofuscin have strong reducing properties, capable of reducing ferric ions to ferrous ions, which then react with potassium ferricyanide to yield a dark blue color. This method detects all substances that can reduce ferric compounds and is therefore not specific for melanin and lipofuscin. However, differentiation can be assisted based on tissue/cell type, pigment location and distribution, and staining intensity. Comparative observations can also be performed alongside methods such as the ammoniacal silver method, aldehyde-fuchsin method, and periodic acid-Schiff (PAS) method.This reagent is intended for research use only and not for clinical diagnosis or other purposes.
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Just before use, mix Solution A and Solution B at a ratio of 9:1 to prepare Schmorl Stain. Do not prepare the stain in advance.
Materials to Be Prepared by the User
Fixatives (10% neutral buffered formalin, 10% formalin, etc.), Graded ethanol solutions, 1% acetic acid aqueous solution
Procedure (For Reference Only)
(1) Paraffin Section Staining
1. Fix tissues in 10% neutral buffered formalin, then process for routine dehydration and embedding.
2. Cut sections at a thickness of 4 μm. Deparaffinize sections with xylene or a deparaffinization solution and hydrate to distilled water; rinse with distilled water for 1 min.
3. Immerse sections in Schmorl stain (see Note 3) for 1-5 min; rinse with distilled water for 2 min.
4. (Optional) Rinse sections in 1% acetic acid aqueous solution for 1-3 min to thoroughly remove residual ferricyanide; rinse with distilled water for 2 min.
5. Counterstain with nuclear counterstain for 3-10 min; rinse with distilled water for 1-2 min.
6. Dehydrate sections routinely, clear with xylene or a deparaffinization solution, and mount with neutral mounting medium.
(2) Frozen Section Staining
1. Skip deparaffinization; rinse sections quickly with distilled water for 2-3 min.
2. Follow the staining and mounting steps for paraffin sections, with appropriate reduction of incubation times.
(3) Cell Staining
1. Fix cells with 4% paraformaldehyde for 10-20 min.
2. Rinse with tap water twice, 2 min each time.
3. Rinse with distilled water twice, 2 min each time.
4. Follow the staining and mounting steps for paraffin sections, with appropriate extension of incubation times.
Staining Results
Lipofuscin, Melanin Greenish: blue to dark blue
Cell Nuclei, Other Tissues: Red
Precautions
1. Ensure complete deparaffinization of sections.
2. Use distilled or deionized water throughout the procedure to avoid interference from reducing impurities in water.
3. Prepare Schmorl stain fresh immediately before use; it is stable for approximately 2 hours. Do not over-incubate sections in the stain. Generally, melanin stains faster than lipofuscin and should show a positive reaction within 2 min. Prolonged staining will cause heavy background coloration, impairing observation contrast.
4. The 1% acetic acid rinse step is optional and helps remove residual ferricyanide.
5. For frozen section and cell staining, optimize experimental conditions according to specific sample types.
6. This method detects all ferric ion-reducing substances and lacks specificity for lipofuscin. Differentiation of lipofuscin can be aided by tissue/cell type, pigment location/distribution, and staining intensity. Comparative observations with the ammoniacal silver method, aldehyde-fuchsin method, and PAS method are recommended.
7. Most tissues exhibit inherent reducing properties that can cause background staining. Therefore, strictly control staining time to achieve clear visualization of lipofuscin with minimal background.
8. For safety and health, wear a lab coat and disposable gloves during operation.
9. Use the reagent promptly after opening to avoid compromising experimental results.
规格
别名
黑色素脂褐素染色液
英文别名
Melanin-Lipofuscin Staining Solution
规格或纯度
BioReagent,用于显微镜,生物染色剂
稳定性与储存
各组分在相应的储存条件下保质期1年。
英文名称
Melanin-Lipofuscin Staining Solution (Schmorl's Method)
储存条件
2-8°C储存,避光,室温
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
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C of A & Other Certificates(BSE/TSE, COO):
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 H2626304 |
分析证书 | A1508695 |
| ZJ26F0332968 |
分析证书 | A1508695 |
技术文档和文章
溶液计算器
常见问题
什么是「生物染色剂」？
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本产品应如何储存？
请于2–8 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
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