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ATP 柠檬酸裂解酶（ATP citrate lyase，ACL）是催化柠檬酸生成乙酰辅酶 A 的关键胞质酶，其催化产生的乙酰辅酶 A 是合成脂肪酸与胆固醇等脂类物质的主要原料，并可参与相关重要蛋白的修饰作用，是体内能源物质代谢的枢纽性物质。该试剂盒可检测动植物组织，细菌和细胞，血清（浆）。在该试剂盒中，在 ATP 和辅酶 A 存在的情况下，ACL 能将柠檬酸催化裂解为乙酰辅酶 A、草酰乙酸、ADP 和磷酸盐，苹果酸脱氢酶进一步催化草酰乙酸和 NADH 生成苹果酸和NAD⁺，导致 340 nm 处光吸收下降。
ATP Citrate Lyase (ACL) is a key cytoplasmic enzyme that catalyzes the formation of acetyl-CoA from citrate. The acetyl-CoA produced serves as the primary precursor for the synthesis of fatty acids, cholesterol, and other lipids, and it is also involved in the modification of important proteins. As such, acetyl-CoA is a central hub in energy metabolism. This assay kit is suitable for detecting ACL activity in samples such as animal and plant tissues, bacteria, cells, and serum (plasma). In this kit, in the presence of ATP and coenzyme A, ACL catalyzes the cleavage of citrate into acetyl-CoA, oxaloacetate, ADP, and inorganic phosphate. Malate dehydrogenase then further catalyzes the reaction of oxaloacetate with NADH to produce malate and NAD⁺, resulting in a decrease in absorbance at 340 nm.
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