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乙醇脱氢酶(Alcohol dehydrogenase，ADH)的系统名为乙醇：辅酶I氧化还原酶（alcohol：NAD⁺ oxidoreductase），大量存在于人和动物肝脏、植物及微生物细胞之中，是一种含锌金属酶，具有广泛的底物特异性。乙醇脱氢酶够以烟酰胺腺嘌呤二核苷酸(NAD)为辅酶，催化伯醇和醛之间的可逆反应： CH3CH2OH+ NAD⁺→ CH3CHO +NADH+ H⁺。 在人和哺乳动物体内，乙醇脱氢酶与乙醛脱氢酶(ALDH)构成了乙醇脱氢酶系，参乙醇脱氢酶与体内乙醇代谢，是人和动物体内重要的代谢酶，作为生物体内主要短链醇代谢的关键酶，它在很多生理过程中起着重要作用；丙酮酸脱羧酶(PDC)、乙醇脱氢酶(ADH)是乙醇发酵途径的关键酶，无氧呼吸途径代谢产物的过程积累对细胞产生毒性，影响线粒体结构和三羧酸循环的相关酶活性。 该试剂盒的检测原理是在弱碱条件下，以乙醛为底物，乙醛在ADH催化下被NADH还原为乙醇，ADH每催化1分子乙醛消耗1分子NADH，通过分光光度比色法(全波长酶标仪)测定340nm处吸光度的变化，计算出NADH的消耗速率进一步推算出乙醇脱氢酶活性水平。主要用于检测植物样本、血清等中乙醇脱氢酶活性。本产品仅用于科研领域，不适用于临床诊断或其他用途。
Alcohol Dehydrogenase (ADH), systematic name alcohol: NAD⁺ oxidoreductase, is a zinc-containing metalloenzyme with broad substrate specificity. It is abundantly present in the livers of humans and animals, as well as in plant and microbial cells. ADH utilizes nicotinamide adenine dinucleotide (NAD⁺) as a coenzyme to catalyze the reversible reaction between primary alcohols and aldehydes: CH₃CH₂OH + NAD⁺ → CH₃CHO + NADH + H⁺. In humans and mammals, ADH together with acetaldehyde dehydrogenase (ALDH) constitutes the alcohol dehydrogenase system, which is involved in ethanol metabolism and plays a vital role as a key metabolic enzyme. As a critical enzyme in the metabolism of short-chain alcohols, ADH is important in numerous physiological processes. Additionally, pyruvate decarboxylase (PDC) and ADH are key enzymes in the ethanol fermentation pathway. The accumulation of metabolites from anaerobic respiration can be toxic to cells, affecting mitochondrial structure and the activity of enzymes related to the tricarboxylic acid (TCA) cycle. Under weakly alkaline conditions, using acetaldehyde as the substrate, ADH catalyzes the reduction of acetaldehyde to ethanol by NADH. Each molecule of acetaldehyde reduced consumes one molecule of NADH. By measuring the change in absorbance at 340 nm using spectrophotometry (full-wavelength microplate reader), the rate of NADH consumption is determined, from which the activity level of alcohol dehydrogenase can be calculated. This kit is primarily used to detect alcohol dehydrogenase activity in samples such as plant tissues and serum. It is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
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