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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
ACL（EC4.1.3.8）是脂肪酸合成中的关键酶，其催化产生的乙酰辅酶A可用于脂肪酸合成和碳链延长、黄酮类物质合成、氨基酸合成等。 测定原理：ACL在ATP和辅酶A存在的情况下催化柠檬酸裂解为乙酰辅酶A、草酰乙酸、腺苷二磷酸和磷酸盐。苹果酸脱氢酶进一步催化草酰乙酸和NADH生成苹果酸和NAD⁺，在340nm测定NADH减少速率，计算ACL活性。
ATP-Citrate Lyase (EC 4.1.3.8) is a key enzyme in fatty acid synthesis. The acetyl-CoA it generates can be utilized for fatty acid synthesis and chain elongation, flavonoid synthesis, amino acid synthesis, and other metabolic processes. Assay Principle: In the presence of ATP and coenzyme A, ACL catalyzes the cleavage of citrate into acetyl-CoA, oxaloacetate, ADP, and inorganic phosphate. Malate dehydrogenase further catalyzes the conversion of oxaloacetate and NADH to malate and NAD⁺. The activity of ACL is calculated by measuring the rate of decrease in NADH absorbance at 340 nm.
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