计算溶液所需的质量、体积或浓度。
BioReagent,用于蛋白分析,用于蛋白测序 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
氨基黑10B可用于检测印迹膜上的蛋白,转移的蛋白大于50ng/条带时,在淡蓝色的背景上可呈现深蓝色的条带,在测定内部序列时,对于蛋白质测序和蛋白质原位切割,氨基黑是最常用的染色剂。
氨基黑10B染色漂洗液主要由甲醇、弱酸、去离子水等组成,适用于氨基黑10B染色液(0.1%-0.5%)的染色后漂洗。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料
操作步骤(仅供参考)
1. 将印迹转移膜放在恰当容器中,蒸馏水水洗3次,每次5 min。
2. 浸入氨基黑10B染色液 (0.1%) 染色5 min或更长时间,染色过程中不时晃动染色皿。
3. 准备3个漂洗皿,加入氨基黑10B染色漂洗液,从染色液中取出薄膜条并尽量沥去染色液,按顺序投入漂洗液中反复漂洗,直至背景无色为止。
4. 对结果作适当记录或拍照。
注意事项
1. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
2. 试剂开封后请尽快使用,以防影响后续实验效果。
Amido Black 10B can be used to detect proteins on blotting membranes. When the transferred protein is greater than 50 ng per band, dark blue bands appear on a light blue background. Amido Black is the most commonly used stain for protein sequencing and in situ protein cleavage when determining internal sequences.
Amido Black 10B Staining Washing Solution is mainly composed of methanol, weak acid, deionized water and other components, and is used for washing membranes after staining with Amido Black 10B Staining Solution (0.1%–0.5%).This reagent is for research use only and is not intended for clinical diagnosis or other purposes.
Materials to Prepare
Distilled or deionized water
Amido Black 10B Staining Solution
Shaker
Procedure (For Reference Only)
Place the blotting transfer membrane in a suitable container. Wash with distilled water 3 times, 5 minutes each time.
Immerse the membrane in Amido Black 10B Staining Solution (0.1%) for 5 minutes or longer. Shake the staining dish occasionally during the staining process.
Prepare 3 destaining dishes containing Amido Black 10B Staining Washing Solution. Remove the membrane strip from the staining solution, drain off as much staining solution as possible, and successively immerse it in the washing solutions. Rinse repeatedly until the background is colorless.
Appropriately record or photograph the results.
Precautions
For your safety and health, please wear a lab coat and disposable gloves during operation.
Use the reagent as soon as possible after opening to prevent affecting subsequent experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | A1516108 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 for Protein analysis 级别指南 → 查看 for Protein sequencing 级别指南 →