计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 2 篇同行评审文献引用。
推荐将Annexin V结合缓冲液与Annexin V试剂一起使用。细胞应以1x10 6 / ml的浓度重悬于膜联蛋白V结合缓冲液中。结合缓冲液包含最佳浓度的钙,这是膜联蛋白V与细胞表面磷脂酰丝氨酸(PS)结合所需的最佳浓度。膜联蛋白V是钙依赖性磷脂结合蛋白,其优先结合包括PS在内的带负电荷的磷脂。通过细胞凋亡进行的细胞可以根据其膜联蛋白V和碘化丙啶染色模式进行监测。早期的凋亡细胞将结合膜联蛋白V,但对碘化丙锭(PI)的细胞内染色不敏感。随着细胞通过细胞凋亡前进,质膜的完整性会丧失,从而使PI能够穿透并标
Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x10|6|/ml. The binding buffer contains an optimal concentration of calcium which is required for Annexin V binding to phosphotidylserine (PS) on the cell surface. Annexin V is a calcium dependent phospholipid binding protein that preferentially binds to negatively charged phospholipids including PS. Cells progressing through apoptosis can be monitored according to their Annexin V and propidium iodide staining pattern. Early apoptotic cells will bind Annexin V but are not sensitive to intracellular staining with propidium iodide (PI). As cells progress through apoptosis the integrity of the plasma membrane is lost, allowing PI to penetrate and label the cells with a strong yellow-red fluorescence.
Annexin V Binding Buffer is a optimal formula containing 2.5 mM Calcium Chloride in HEPES buffered saline.Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml.
Annexin V Binding Bufferis a buffer used with Annexin V reagents that is used for flow cytometry and immunofluorescence applications
P264: 处理后要彻底洗手。