Aladdin® 488是一种明亮的绿色荧光染料，其激发光谱非常适合于 488 nm 激光谱线。Aladdin® 488染料用于成像和流式细胞分析中稳定信号的生成，具有水溶性和 pH 值不敏感性（pH 值 4 - pH 值 10）。
Aladdin® 488的 NHS 酯（或琥珀酰亚胺酯）是将该染料与蛋白或抗体偶联的较常用工具。NHS 酯可用于标记蛋白、胺修饰的寡核苷酸和其他含胺分子的伯胺 (R-NH2)。所得偶联物将显示出比其他光谱相似荧光基团的偶联物更亮的荧光和更高的光稳定性。
荧光基团标记：Aladdin® 488染料
反应性基团：NHS 酯
反应性：蛋白和配体、胺修饰的寡核苷酸上的伯胺
偶联物的 Ex/Em：494/517 nm
消光系数：73,000 cm-1M-1
光谱类似染料：FITC、Oregon Green 488
Aladdin® 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line. Used for stable signal generation in imaging and flow cytometry, Aladdin® 488 dye is water soluble and pH-insensitive from pH 4 to pH 10.
The NHS ester (or succinimidyl ester) of Aladdin® 488 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.
Fluorophore label: Aladdin® 488 dye
Reactive group: NHS ester
Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides
Ex/Em of the conjugate: 494/517 nm
Extinction coefficient: 73,000 cm-1M-1
Spectrally similar dyes: FITC, Oregon Green 488
G2511143
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