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BioReagent,细菌学等级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
发根农杆菌是根瘤菌科(Rhizobiaceae)农杆菌属(agrobacterium)的一种革兰氏阴性土壤细菌,它能够感染大多数双子叶植物和少数单子叶植物以及个别裸子植物。Ar.A4发根农杆菌菌株含有农杆碱型Ri质粒,具有广泛的宿主范围(玉米,烟草,胡萝卜,甘草等),同时具有卡那霉素抗性。
产品用途:发根农杆菌菌株
原核抗性: Kan+
基因型: Agrobacterium rhizogenes(kanR) Ar A4 Ri(agropine type)
培养基: TY 培养基
培养条件: 30℃,有氧
产品规格:200μL 甘油菌
菌株扩增: 取100-200μl甘油菌,接种至3-5ml新鲜TY培养基(Kan+抗性)中,30°C过夜摇菌即可。也可以先用甘油菌划板,然后挑单克隆,再过夜摇菌。
Agrobacterium rhizogenes is a Gram-negative soil bacterium belonging to the genus Agrobacterium in the family Rhizobiaceae. It can infect most dicotyledonous plants, a few monocotyledonous plants, and some gymnosperms. The Agrobacterium rhizogenes strain Ar.A4 contains an agropine-type Ri plasmid, has a broad host range (including maize, tobacco, carrot, Glycyrrhiza uralensis, etc.), and is resistant to kanamycin.
Product Usage: Agrobacterium rhizogenes strain
Prokaryotic Resistance: Kan+
Genotype: Agrobacterium rhizogenes(kanR) Ar A4 Ri(agropine type)
Culture Medium: TY Medium
Culture Conditions: 30°C, aerobic
Product Specification: 200 μL Glycerol Stock
Strain Amplification: Take 100–200 μL of the glycerol stock, inoculate it into 3–5 mL of fresh TY medium (with Kan+ resistance), and incubate with shaking overnight at 30°C. Alternatively, you can first streak the glycerol stock on an agar plate, then pick a single colony, and incubate with shaking overnight.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | A743858 |
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