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BioReagent,细菌学等级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
GV3101根瘤农杆菌为C58型背景,核基因中含有筛选标签---利福平抗性基因Rif。为了便于转化操作,此菌株携带一无自身转运功能的胭脂碱型Ti质粒pMP90 (pTiC58DT-DNA),此质粒含有vir基因,vir基因是T-DNA插入植物基因组必需的元件,pMP90(pTiC58DT-DNA)质粒自身的T-DNA转移功能被破坏,但可以帮助转入的双元载体T-DNA顺利转移。pMP90 (pTiC58DT-DNA)型Ti质粒含有gent筛选标记,赋予GV3101菌株庆大霉素抗性,适用于拟南芥、烟草、玉米、土豆等植物的转基因操作。
产品用途:根瘤农杆菌菌株
原核抗性:Rif+、Gen+
基因型:C58 (RifR) Ti pMP90 (pTiC58DT-DNA) (GentR) Nopaline
培养基: LB培养基
培养条件: 28-30℃,有氧
产品规格:200μL 甘油菌
菌株扩增:取100-200μl甘油菌,接种至3-5ml新鲜LB培养基(Rif+、Gen+双抗性)中,28-30°C过夜摇菌即可。也可以先用甘油菌划板,然后挑单克隆,再过夜摇菌。
Agrobacterium tumefaciens GV3101 has a C58-type background, with a screening marker—the rifampicin resistance gene Rif—present in its nuclear genome. To facilitate transformation operations, this strain carries a nopaline-type Ti plasmid pMP90 (pTiC58ΔT-DNA) that lacks self-transport functionality. This plasmid contains vir genes, which are essential components for the insertion of T-DNA into the plant genome. Although the T-DNA transfer function of the pMP90 (pTiC58ΔT-DNA) plasmid itself is disrupted, it can assist in the smooth transfer of T-DNA from the introduced binary vector. The pMP90 (pTiC58ΔT-DNA)-type Ti plasmid contains a gent screening marker, which endows the GV3101 strain with gentamicin resistance and is suitable for transgenic operations in plants such as Arabidopsis thaliana, tobacco, maize, and potato.
Product Usage: Agrobacterium tumefaciens strain
Prokaryotic Resistance: Rif+、Gen+
Genotype: C58 (RifR) Ti pMP90 (pTiC58DT-DNA) (GentR) Nopaline
Culture Medium: LB Medium
Culture Conditions: 28–30°C, aerobic
Product Specification: 200 μL Glycerol Stock
Strain Amplification: Take 100–200 μL of the glycerol stock, inoculate it into 3–5 mL of fresh LB medium (with Rif+ and Gen+ double resistance), and incubate with shaking overnight at 28–30°C. Alternatively, you can first streak the glycerol stock on an agar plate, then pick a single colony, and incubate with shaking overnight.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | G746577 |