计算溶液所需的质量、体积或浓度。
BioReagent,细菌学等级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-80℃储存,避免反复冻融。特低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
发根农杆菌是根瘤菌科(Rhizobiaceae)农杆菌属(agrobacterium)的一种革兰氏阴性土壤细菌,它能够感染大多数双子叶植物和少数单子叶植物以及个别裸子植物。C58C1发根农杆菌菌株含有pRiA4b农杆碱型Ri质粒,具有广泛的宿主范围(蔷薇科,夹竹桃科,豆科,茄科,黄芩,烟草等),同时具有链霉素,利福平抗性。
产品用途:发根农杆菌菌株
原核抗性:Str+、Rif+
基因型: Agrobacterium rhizogenes (strR, rifR) pRiA4b (agropine type)
培养基: LB培养基
培养条件: 28-30℃,有氧
产品规格:200μL 甘油菌
菌株扩增: 取100-200μl甘油菌,接种至3-5ml新鲜LB培养基(Str+、Rif+抗性)中,28-30°C过夜摇菌即可。也可以先用甘油菌划板,然后挑单克隆,再过夜摇菌。
Agrobacterium rhizogenes is a Gram-negative soil bacterium belonging to the genus Agrobacterium of the family Rhizobiaceae. It can infect most dicotyledonous plants, a few monocotyledonous plants, and some gymnosperms. The Agrobacterium rhizogenes strain C58C1 contains the agropine-type Ri plasmid pRiA4b, has a broad host range (Rosaceae, Apocynaceae, Fabaceae, Solanaceae, Scutellaria baicalensis, Nicotiana tabacum, etc.), and is resistant to streptomycin and rifampicin.
Product Usage: Agrobacterium rhizogenes strain
Prokaryotic Resistance: Str+、Rif+
Genotype: Agrobacterium rhizogenes (strR, rifR) pRiA4b (agropine type)
Culture Medium: LB Medium
Culture Conditions: 28–30°C, aerobic
Product Specification: 200 μL Glycerol Stock
Strain Amplification: Take 100–200 μL of the glycerol stock, inoculate it into 3–5 mL of fresh LB medium (with Str+ and Rif+ resistance), and incubate with shaking overnight at 28–30°C. Alternatively, you can first streak the glycerol stock on an agar plate, then pick a single colony, and incubate with shaking overnight.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C744347 |