阿利新蓝 (Alcian)又称爱先蓝或阿尔辛蓝等，是一种类铜钛花青共轭染料，最初用于纺织纤维染色。这种阳离子染料与酸性基团结合，也即阿尔辛蓝与组织内含有的阴离子基团如羧基和硫酸根形成不溶性复合物。阿利新蓝由中央含铜的酞菁环与四个异硫脲基通过硫醚键相连而成，该异硫脲基呈中度碱性，使阿利新蓝带阳离子。pH 值为2.5 时，组织内的羧基电离，带有一个负电荷，与阿利新蓝中的阳离子形成盐键，使带有羧基的酸性黏液物质(硫酸黏蛋白和唾液黏蛋白)染色。

阿利新蓝染色液 (pH2.5)又称标准阿利新蓝染色液，利用染液的不同pH值可区分粘液物质的类属，pH为1时，羧基 (COOH)不着色，硫酸基 (OSO3H)着色，pH为2.5时，羧基染色良好而硫酸粘液着色不佳，使硫酸黏蛋白和唾液黏蛋白着色。中性黏蛋白 (如胃黏膜和Brunner腺体部位的中性黏蛋白)不能与阿利新蓝反应。本试剂仅适用于科研领域，不适用于临床诊断或其他用途。

A774858	Component	3×50 mL	3×100 mL	Storage
A774858A	阿利新蓝酸化液	50 mL	100 mL	RT.
A774858B	阿利新蓝染色液	50 mL	100 mL	RT. Store in the dark
A774858C	核固红染色液	50 mL	100 mL	RT. Store in the dark

自备材料：

1、系列乙醇

2、蒸馏水

操作步骤(仅供参考)：

1、二甲苯脱蜡，通过梯度乙醇后，入蒸馏水再水化。

2、入阿利新蓝酸化液浸泡5min。

3、入阿利新蓝染色液浸染30min。

4、流水冲洗。

5、入核固红染色液，复染5-10min。流水冲洗。

6、梯度乙醇脱水，二甲苯透明，中性树胶封片。

染色结果:

酸性黏蛋白 (硫酸黏蛋白和唾液黏蛋白): 蓝色

蛋白多糖和透明质酸: 蓝色

细胞核: 红色

注意事项：

1、固定液采用10%中性福尔马林。

2、若要选择性鉴别硫酸黏蛋白和蛋白多糖，应使用pH值低 (pH=1.0)的阿利新蓝，即调pH值为1.0。染色程序与pH=2.5的阿利新蓝操作相同，染色时间应相应延长。

3、已开封试剂应在开封后6个月内使用完，每次用后应及时拧紧瓶盖，以免挥发或变质。

4、为了您的安全和健康，请穿实验服并戴一次性手套操作。

5、本产品仅供科研使用，严禁它用。

Alcian Blue, also known as Alician Blue or Alcyan Blue, is a copper - phthalocyanine conjugated dye originally used for textile fiber dyeing. As a cationic dye, it binds to acidic groups, namely, it forms insoluble complexes with anionic groups such as carboxyl groups and sulfate groups contained in tissues. Alcian Blue is composed of a central copper - containing phthalocyanine ring linked to four isothiourea groups via thioether bonds. The isothiourea groups are moderately basic, endowing Alcian Blue with cationic properties. At pH 2.5, carboxyl groups in tissues ionize and carry a negative charge, forming salt bonds with the cations in Alcian Blue, thus staining acidic mucous substances (sulfomucins and sialomucins) with carboxyl groups.

Alcian Blue Staining Solution (pH 2.5) is also called standard Alcian Blue Staining Solution. Different pH values of the staining solution can be used to distinguish the types of mucous substances. At pH 1, carboxyl groups (COOH) are not stained while sulfate groups (OSO₃H) are stained. At pH 2.5, carboxyl groups are well - stained but sulfated mucins are poorly stained, resulting in the staining of sulfomucins and sialomucins. Neutral mucins (such as those in gastric mucosa and Brunner's glands) do not react with Alcian Blue. This reagent is only applicable to scientific research and not for clinical diagnosis or other purposes.

A774858	Component	3×50 mL	3×100 mL	Storage
A774858A	Alcian Blue Acidification Solution	50 mL	100 mL	RT.
A774858B	Alcian Blue Staining Solution	50 mL	100 mL	RT. Store in the dark
A774858C	Nuclear Fast Red Staining Solution	50 mL	100 mL	RT. Store in the dark

Materials to Be Prepared by Users

1. Graded ethanol solutions

2. Distilled water

Operating Procedures (for reference only)

1. Deparaffinize the sections with xylene, rehydrate them through graded ethanol solutions, and then transfer them into distilled water.

2. Immerse the sections in Alcian Blue Acidification Solution for 5 minutes.

3. Stain the sections in Alcian Blue Staining Solution for 30 minutes.

4. Rinse the sections with running water.

5. Counterstain the sections in Nuclear Fast Red Staining Solution for 5-10 minutes, followed by rinsing with running water.

6. Dehydrate the sections with graded ethanol solutions, clear them with xylene, and mount the sections with neutral balsam.

Staining Results

Acidic mucins (sulfomucins and sialomucins): Blue

Proteoglycans and hyaluronic acid: Blue

Cell nuclei: Red

Precautions

1. Use 10% neutral formalin as the fixative.

2. For the selective identification of sulfomucins and proteoglycans, Alcian Blue with a low pH value (pH=1.0) should be used, i.e., adjust the pH value to 1.0. The staining procedure is the same as that for Alcian Blue at pH=2.5, but the staining time should be appropriately extended.

3. Opened reagents should be used up within 6 months after opening. Tighten the bottle caps immediately after each use to avoid volatilization or deterioration.

4. For your safety and health, wear a lab coat and disposable gloves during operation.

5. This product is for research use only and shall not be used for other purposes.

规格

别名

阿利新蓝染色液 | 爱先蓝染色液 | 阿尔辛蓝染色液

英文别名

Alcian Blue Staining Solution

规格或纯度

BioReagent, 生物染色剂, 用于显微镜

稳定性与储存

各组分在相应的储存条件下保质期1年。

英文名称

Alcian Blue Staining Solution (pH 2.5)

储存条件

避光,室温

运输条件

常规运输

产品线

生物产品

名称和识别符

分子类型

生物试剂/缓冲液

高级数据

产品属性

pH	2.5

化学和物理性质

敏感性

Light-sensitive

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ26F0434542	分析证书	A774858
ZJ26F0131764	分析证书	A774858
ZJ26F0131765	分析证书	A774858

技术文档和文章

a774858_阿利新蓝染色液ph2.5产品说明书.pdf

a774858_alcian_blue_staining_solution_ph_2_5_instruction_manual.pdf

生物染色剂

目录：阿拉丁试剂的规格、级别和纯度

探索主题:

级别生物染色剂

Biological Stain

目录：阿拉丁试剂的规格、级别和纯度

探索主题:

grade Biological Stain

涂片染色、微生物学染色与基础染料体系的原理与方法

目录：技术文章

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涂片染色微生物学染色

Principles and methods of smear staining, microbiological staining, and fundamental dye systems

目录：技术文章

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Microbiological staining Smear staining

软骨基质染色中番红O、阿利新蓝与甲苯胺蓝的应用区别

目录：技术文章

探索主题:

组织化学染色阿利新蓝甲苯胺蓝糖胺聚糖番红O 骨关节炎蛋白多糖软骨基质酸性黏多糖异染性软骨退变成软骨分化

Application Differences Among Safranin O, Alcian Blue, and Toluidine Blue in Cartilage Matrix Staining

目录：技术文章

探索主题:

Alcian Blue Toluidine Blue Glycosaminoglycans Proteoglycans Safranin O osteoarthritis histochemical staining cartilage matrix acidic mucopolysaccharide metachromasia cartilage degeneration chondrogenic differentiation

结缔组织染色的组织成分识别、方法选择与结果判读

目录：技术文章

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Masson三色染色 PAS染色阿利新蓝纤维化胶原纤维 Van Gieson染色天狼星红染色软骨基质结缔组织染色网状纤维染色弹力纤维染色 Verhoeff染色矿化基质弹力纤维

Identification of Tissue Components, Method Selection, and Result Interpretation in Connective Tissue Staining

目录：技术文章

探索主题:

PAS Staining Alcian Blue fibrosis Masson’s trichrome staining Van Gieson staining Sirius red staining Collagen Fibers cartilage matrix connective tissue staining reticular fiber staining elastic fiber staining Verhoeff staining mineralized matrix elastic fibers

尿沉渣显微镜检查的形态学基础、方法流程与结果判读

目录：技术文章

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细菌白细胞尿沉渣显微镜检查红细胞上皮细胞管型尿结晶真菌寄生虫黏液丝相差显微镜尿液有形成分

Morphological Basis, Method Workflow, and Result Interpretation of Urinary Sediment Microscopy

目录：技术文章

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Bacteria urinary sediment microscopy red blood cells white blood cells epithelial cells casts urinary crystals fungi parasites mucus threads phase-contrast microscopy formed elements in urine

Russell改良Movat五色套染法动脉粥样硬化斑块及结缔组织成分综合显示实验操作规程

目录：实验方案（Protocols）

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Movat五色套染法

Experimental Protocol for the Russell Modified Movat Pentachrome Method for Comprehensive Visualization of Atherosclerotic Plaques and Connective Tissue Components

目录：实验方案（Protocols）

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Movat Pentachrome Method

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

需要帮助选择级别？

我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。

查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →

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阿利新蓝染色液 (pH2.5)

概述

规格

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

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