计算溶液所需的质量、体积或浓度。
阿利新蓝染色液（pH 1.0）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
阿利新蓝（Alcian）又称爱先蓝或阿尔辛蓝等，是一种类铜钛花青共轭染料，最初用于纺织纤维染色。这种阳离子染料与酸性基团结合，也即阿尔辛蓝与组织内含有的阴离子基团如羧基和硫酸根形成不溶性复合物。阿利新蓝由中央含铜的酞菁环与四个异硫脲基通过硫醚键相连而成，该异硫脲基呈中度碱性，使阿利新蓝带阳离子。pH值为2.5 时，组织内的羧基电离，带有一个负电荷，与阿利新蓝中的阳离子形成盐键，使带有羧基的酸性黏液物质（硫酸黏蛋白和唾液黏蛋白）染色。
阿利新蓝染色液（pH 1.0）利用染液的不同pH值可区分粘液物质的类属，pH为1时，羧基（COOH）不着色，硫酸基（OSO₃H）着色，pH为2.5时，羧基染色良好而硫酸粘液着色不佳。pH 值为1.0时，组织 内的硫酸根电离，带有一个负电荷，与阿利新蓝中的阳离子形成盐键，使带有硫酸根的组织（如硫酸黏液物质）染色。中性黏蛋白（如胃黏膜和 Brunner 腺体部位的中性黏蛋白）不能与阿利新蓝反应。
| A774857 | Component | 3×50 mL | 3×100 mL | Storage |
| A774857A | 阿利新蓝酸化液 | 50 mL | 100 mL | RT. |
| A774857B | 阿利新蓝染色液 | 50 mL | 100 mL | RT. Store in the dark |
| A774857C | 核固红染色液 | 50 mL | 100 mL | RT. Store in the dark |
自备材料
系列乙醇、蒸馏水
使用说明
1. 二甲苯脱蜡，通过梯度乙醇后，入蒸馏水再水化。
2. 入阿利新蓝酸化液浸泡5 min。
3. 入阿利新蓝染色液浸染30 min。流水冲洗。
4. 阿利新蓝酸化液稍洗。
5. 不经水洗，直接用滤纸吸干多余的阿利新蓝酸化液。
6. 入核固红染色液，复染。流水冲洗。
7. 常规乙醇脱水，二甲苯透明，中性树胶封片。
注意事项
1. 固定液采用10%中性福尔马林。
2. 该法可区分鉴别硫酸黏蛋白和蛋白多糖，其pH=1.0。
3. 已开封试剂应在开封后6个月内使用完，每次用后应及时拧紧瓶盖，以免挥发或变质。
4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
5. 本产品仅供科研使用，严禁它用。
染色结果
| 硫酸黏蛋白 | 蓝色 |
| 非硫酸化酸性黏液物质 | 不着色 |
| 细胞核 | 浅红色 |
Alcian Blue (also known as Alcian blue) is a copper phthalocyanine conjugated dye originally used for textile fiber dyeing. This cationic dye binds to acidic groups, i.e., Alcian blue forms insoluble complexes with anionic groups in tissues such as carboxyl and sulfate groups. Alcian blue consists of a central copper‑containing phthalocyanine ring linked via thioether bonds to four isothiouronium groups. These isothiouronium groups are moderately alkaline, giving Alcian blue its cationic character. At pH 2.5, carboxyl groups in tissues ionize, carrying a negative charge, and form salt bonds with the cations in Alcian blue, thereby staining acidic mucus substances containing carboxyl groups (sulfated mucins and sialomucins).
Alcian Blue Staining Solution (pH 1.0) utilizes different pH levels of the dye to distinguish classes of mucus substances. At pH 1.0, carboxyl groups (COOH) do not stain, while sulfate groups (OSO₃H) stain. At pH 2.5, carboxyl groups stain well but sulfated mucins stain poorly. At pH 1.0, sulfate groups in tissues ionize, carrying a negative charge, and form salt bonds with the cations in Alcian blue, staining tissues containing sulfate groups (e.g., sulfated mucus substances). Neutral mucins (such as neutral mucins in gastric mucosa and Brunner’s glands) do not react with Alcian blue.
| A774857 | Component | 3×50 mL | 3×100 mL | Storage |
| A774857A | Alcian Blue Acidification Solution | 50 mL | 100 mL | RT. |
| A774857B | Alcian Blue Staining Solution | 50 mL | 100 mL | RT. Store in the dark |
| A774857C | Nuclear Fast Red Staining Solution | 50 mL | 100 mL | RT. Store in the dark |
Materials to Be Prepared by User
Graded ethanol series, distilled water
Instructions for Use
Deparaffinize in xylene, rehydrate through a graded ethanol series, and rinse in distilled water.
Immerse in Alcian Blue Acidification Solution for 5 minutes.
Stain in Alcian Blue Staining Solution for 30 minutes. Rinse under running water.
Rinse briefly with Alcian Blue Acidification Solution.
Without water rinse, blot off excess Alcian Blue Acidification Solution using filter paper.
Counterstain in Nuclear Fast Red Staining Solution. Rinse under running water.
Dehydrate routinely through ethanol, clear in xylene, and mount with neutral balsam.
Precautions
Use 10% neutral formalin as fixative.
This method can distinguish sulfated mucins from proteoglycans; its pH = 1.0.
Opened reagents should be used within 6 months after opening. Tighten the cap immediately after each use to prevent evaporation or deterioration.
For your safety and health, please wear a lab coat and disposable gloves during operation.
This product is for research use only; any other use is strictly prohibited.
Staining Results
|
规格
别名
阿尔新蓝染色液 | 阿尔新蓝-核固红染色液 | 爱先蓝染色液
英文别名
Alcian Blue & Nuclear Fast Red Staining Kit, pH1.0 | Alnin Blue–Nuclear fast red Staining Kit
规格或纯度
BioReagent, 生物染色剂, 用于显微镜
稳定性与储存
Store at room temperature long term (6 months). Store in the dark.
英文名称
Alcian Blue Staining Solution (pH 1.0)
储存条件
避光,室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
产品属性
| pH | 1.0 |
|---|
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333317 |
分析证书 | A774857 |
技术文档和文章
生物染色剂
目录： 阿拉丁试剂的规格、级别和纯度
Principles and methods of smear staining, microbiological staining, and fundamental dye systems
目录： 技术文章
Application Differences Among Highman, Bennhold, Puchtler, and Modified Methods in Congo Red Staining for Amyloid
目录： 技术文章
Application Differences Among Safranin O, Alcian Blue, and Toluidine Blue in Cartilage Matrix Staining
目录： 技术文章
Identification of Tissue Components, Method Selection, and Result Interpretation in Connective Tissue Staining
目录： 技术文章
Morphological Basis, Method Workflow, and Result Interpretation of Urinary Sediment Microscopy
目录： 技术文章
Experimental Protocol for the Russell Modified Movat Pentachrome Method for Comprehensive Visualization of Atherosclerotic Plaques and Connective Tissue Components
目录： 实验方案（Protocols）
溶液计算器
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