Rat anti Mouse CD8 alpha Antibody, Monoclonal (YTS 169.4), could be used for in vivo CD8+ T cell depletion, WB, Flow and so on. Application: in vivo CD8+ T cell depletion: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Flow: Use at an assay dependent concentration. Protein Function Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
CD8 alpha Rat mAb (Ab095356) - Flow Cytometry Two-color flow cytometric analysis of CD8 alpha expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or CD8 alpha Rat mAb (Ab095356; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD8 alpha (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.
CD8 alpha Rat mAb (Ab095356) - Flow Cytometry Flow Cytometry analysis of mouse splenocytes cells labelling CD8 alpha with CD8 alpha Rat mAb (Ab095356) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).
CD8 alpha Rat mAb (Ab095356) - Flow Cytometry Flow Cytometry analysis of mouse splenocytes cells labelling CD8 alpha (red) with CD8 alpha Rat mAb (Ab095356) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ24F0809291
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