Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
宿主种属
大鼠(Rat)
特异性
Mouse CD8α
种属反应性
小鼠(Mouse)
免疫原
Mouse Spleen Cells or Thymocyte Membranes
阳性对照
Flow Cytometry: Mouse Splenocyte
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.4
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
图片
Recombinant CD8 alpha Antibody (Ab210971) - Flow Cytometry Flow Cytometry analysis of BALB/c mouse splenocytes labelling CD8 alpha with Recombinant CD8 alpha Antibody (Ab210971) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG (Ab180310) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).
Recombinant CD8 alpha Antibody (Ab210971) - Flow Cytometry Flow Cytometry analysis of BALB/c mouse splenocytes labelling CD8 alpha (red) with Recombinant CD8 alpha Antibody (Ab210971) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG (Ab180310) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD8 alpha Antibody (Ab210971) - SEC The purity of Recombinant CD8 alpha Antibody (Ab210971) is more than 95% verified by HPLC.
ZJ25F0928005
危险品化学品经营许可证（带存储）