Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry, WB, IHC-F, IP, Blocking, ELISA, Functional Assay, Animal Model and so on.
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
宿主种属
大鼠(Rat)
特异性
CD86
种属反应性
小鼠(Mouse)
免疫原
LPS-activated CBA/Ca mouse splenic B cells
阳性对照
Flow: Mouse splenocytes.
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
GL1
Format
Whole IgG
亚型
Rat IgG2a
轻链亚型
kappa
纯化方法
Protein G purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, pH 7.2-7.4
防腐剂
No
储存条件
2-8°C储存,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
Store at 2-8°C. Store at -20°C for long term. Upon delivery aliquot. Avoid freeze/thaw cycle.
图片
CD86 Rat mAb (Ab095462) - Flow Cytometry Flow Cytometry analysis of stimulated with LPS (L118716) for 24h mouse splenocytes cells labelling CD86 (red) with CD86 Rat mAb (Ab095462) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
CD86 Rat mAb (Ab095462) - Flow Cytometry Flow Cytometry analysis of stimulated with LPS (L118716) for 24h mouse splenocytes cells labelling CD86 with CD86 Rat mAb (Ab095462) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Isotype Control (left panel) and detected with SA-PE (rp156150).