Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
宿主种属
大鼠(Rat)
特异性
CD86
种属反应性
小鼠(Mouse)
免疫原
LPS-activated CBA/Ca mouse splenic B cells
阳性对照
Flow: C57BL/6 mouse splenocytes
偶联
AF700
Excitation(nm)
Red Laser(633nm)
Emission(nm)
719nm
Ex/Em(nm)
Ex:696nm;Em:719nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
CD86 Rat mAb (AF700) (Ab215325) - Flow Cytometry LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) stained with CD86 Rat mAb (AF700) (Ab215325) (red) compared to an isotype control (blue). Blue - Rat IgG (AF700) (Ab190242). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).
CD86 Rat mAb (AF700) (Ab215325) - Flow Cytometry LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) stained with CD86 Rat mAb (AF700) (Ab215325) (right panel) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF700) (Ab190242).
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
ZJ25F0420077
危险品化学品经营许可证（带存储）