Goat Anti-Mouse IgG H&L (AF750)

已验证

ExactAb™

1.0 mg/mL

应用:

Flow Cytometry
IF/ICC

功能和特点

宿主种属: 山羊(Goat)
种属反应性: 小鼠(Mouse)
亚型: Goat IgG
偶联: AF750

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab190480-10μg	10μg	期货
Ab190480-100μg	100μg	期货
Ab190480-1mg	1mg	期货

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AF750 (1) Goat Anti-Mouse IgG H&L Antibody (8)

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基本信息

产品名称	Goat Anti-Mouse IgG H&L (AF750)
规格或纯度	ExactAb™, 已验证, 1.0 mg/mL
宿主种属	山羊(Goat)
种属反应性	小鼠(Mouse)
交叉反应	Reacting with mouse whole IgG (mouse IgG light chain, heavy chain), may cross react with immunoglobulins from other species.
免疫原	Mouse IgG
偶联	AF750
Excitation(nm)	Red Laser(633nm)
Emission(nm)	776nm
Ex/Em(nm)	Ex:752nm;Em:776nm

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
纯化方法	Antigen affinity chromatography
储存缓冲液	Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	1.0 mg/mL
储存温度	避光,-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
分子类型	抗体

图片

IF: Goat Anti-Mouse IgG H&L (AF750) (Ab190480)

Goat Anti-Mouse IgG H&L (AF750) (Ab190480) - IF
IF analysis of beta Tubulin (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab179922) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF750) (Ab190480) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Mouse IgG H&L (AF750) (Ab190480)

Goat Anti-Mouse IgG H&L (AF750) (Ab190480) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608). Goat Anti-Mouse IgG H&L (AF750) (Ab190480) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

应用