计算溶液所需的质量、体积或浓度。
Goat Anti-Mouse IgG H&L (AF405) (Ab181948) - IF
IF analysis of beta Tubulin (blue) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab179922) at 1/1500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF405) (Ab181948) at a dilution of 1/500 for 1 hour at room temperature in the dark (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.