Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse IgG Antibody | Anti Mouse I
规格或纯度
ExactAb™, 已验证, 1.0 mg/mL
宿主种属
山羊(Goat)
种属反应性
小鼠(Mouse)
交叉反应
Reacting with mouse whole IgG (mouse IgG light chain, heavy chain), may cross react with immunoglobulins from other species.
免疫原
Mouse IgG
偶联
Oregon Green 488
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
526nm
Ex/Em(nm)
Ex:498nm;Em:526nm
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
Antigen affinity chromatography
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
图片
Goat Anti-Mouse IgG H&L (Oregon Green 488) (Ab217315) - IF IF analysis of beta Tubulin (green) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab179922) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Oregon Green 488) (Ab217315) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Mouse IgG H&L (Oregon Green 488) (Ab217315) - Flow Cytometry Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608). Goat Anti-Mouse IgG H&L (Oregon Green 488) (Ab217315) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ25F0521338
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