Goat Anti-Human IgG Fc (AF647)

已验证

ExactAb™

1.0 mg/mL

应用:

Flow Cytometry
IF/ICC

功能和特点

宿主种属: 山羊(Goat)
种属反应性: 人(Human)
亚型: Goat IgG
偶联: AF647

有货

库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab216756-10μg (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！	10μg	现货
Ab216756-100μg	100μg	现货
Ab216756-1mg	1mg	现货

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AF647 (108) Goat Anti-Human IgG Fc Antibody (11)

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基本信息

产品名称	Goat Anti-Human IgG Fc (AF647)
规格或纯度	ExactAb™, 已验证, 1.0 mg/mL
宿主种属	山羊(Goat)
种属反应性	人(Human)
交叉反应	No cross reactivity with IgM, IgA.
免疫原	Human IgG Fc
偶联	AF647
Excitation(nm)	Red Laser(633nm)
Emission(nm)	668nm
Ex/Em(nm)	Ex:650nm;Em:668nm

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
SDS-PAGE	150kDa
纯化方法	antigen affinity chromatography
储存缓冲液	Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	1.0 mg/mL
储存温度	避光,-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
分子类型	抗体

图片

IF: Goat Anti-Human IgG Fc (AF647) (Ab216756)

Goat Anti-Human IgG Fc (AF647) (Ab216756) - IF
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG Fc (AF647) (Ab216756) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Human IgG Fc (AF647) (Ab216756)

Goat Anti-Human IgG Fc (AF647) (Ab216756) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD20 (red) with Ibritumomab (anti-CD20) (Ab191926). Goat Anti-Human IgG Fc (AF647) (Ab216756) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

应用