Goat Anti-Human IgG Fc Antibody, Secondary antibody, could be used for Flow Cytometry, IF and so on.
规格
产品名称
Goat Anti-Human IgG Fc (Cy5)
别名
羊抗人IgG Fc抗体 | 羊抗人IgG Fc | 山羊抗人IgG Fc | 山羊抗人IgG Fc抗体
英文别名
Goat Anti-Human IgG Fc Antibody | Goat Anti Human IgG Fc Antibody | Anti-Human IgG Fc Antibody | Anti Human IgG Fc Antibody
规格或纯度
ExactAb™, 已验证, 1.0 mg/mL
宿主种属
山羊(Goat)
种属反应性
人(Human)
交叉反应
No cross reactivity with IgM, IgA.
免疫原
Human IgG Fc
偶联
Cy5
Excitation(nm)
Red Laser(633nm)
Emission(nm)
665nm
Ex/Em(nm)
Ex:647nm;Em:665nm
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
Antigen affinity chromatography
浓度
1.0 mg/mL
分子类型
抗体
产品线
biological_products
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
图片
Goat Anti-Human IgG Fc (Cy5) (Ab216761) - IF IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG Fc (Cy5) (Ab216761) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Human IgG Fc (Cy5) (Ab216761) - Flow Cytometry Flow Cytometry analysis of Raji cells labelling CD20 (red) with Ibritumomab (anti-CD20) (Ab191926). Goat Anti-Human IgG Fc (Cy5) (Ab216761) at a dilution of 1/900 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ26F0636106
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