Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.
宿主种属
小鼠(Mouse)
特异性
CEP55
种属反应性
人(Human)
免疫原
Recombinant protein of human CEP55 (AA 1-280).
阳性对照
WB: MCF7 and HeLa cell lysates. IF/ICC: HeLa cells. Flow Cytometry: HeLa cells.
偶联
Unconjugated
克隆类型
单克隆抗体
Format
Whole IgG
纯化方法
Protein A/G purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.4
防腐剂
No
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃（12个月）；收货后建议分装，避免反复冻融。
图片
CEP55 Mouse mAb (Ab336926) - Western Blot
Centrosomal protein 55 expression in wild-type (WT) and centrosomal protein 55 (CEP55) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CEP55 Mouse mAb (Ab336926) (1/2500) and Goat Anti-Mouse IgG H&L (HRP) (Ab179001) (1/20000) respectively.
CEP55 Mouse mAb (Ab336926) - IF/ICC
Immunofluorescent analysis of HeLa cells using CEP55 Mouse mAb (Ab336926) at 1/1000 dilution. Top panel: wild-type (WT); Bottom panal: CEP55 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Centrosomal protein 55 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1xPBS; Permeabilization solution: 0.5% Triton X-100 in 1xPBS. Scale bar, 20 μm.
CEP55 Mouse mAb (Ab336926) - Flow Cytometry
Flow cytometric analysis of Centrosomal protein 55 expression in HeLa cells using CEP55 Mouse mAb (Ab336926) at 1/1000 dilution. Fixative solution: 4% Paraformaldehyde in 1xPBS; Permeabilization solution: 0.5% Triton X-100 in 1xPBS. Green, isotype control; red, Centrosomal protein 55.
CEP55 Mouse mAb (Ab336926) - Western Blot
All lanes: CEP55 Mouse mAb (Ab336926) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
ZJ26F0636811
危险品化学品经营许可证（带存储）