Rabbit anti Human MGEA5/OGA Antibody, Recombinant, could be used for WB, IF/ICC and so on. Application: WB: 1/1000-1/5000 IF/ICC: 1/50-1/200 Protein Function: Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
规格
产品名称
Recombinant MGEA5/OGA Antibody
别名
MGEA5抗体 | MGEA5重组兔单克隆抗体 | OGA抗体 | OGA重组兔单克隆抗体
规格或纯度
重组, ExactAb™, 已验证, 高性能, PBS Only, 见COA
生化机理
Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
宿主种属
兔(Rabbit)
特异性
OGA
种属反应性
人(Human)
免疫原
A synthetic peptide derived from human MGEA5 (AA 2-63)
阳性对照
WB: HeLa, HEK293, HaCaT, K562 lysates. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
PBS, pH 7.4
防腐剂
No
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融
图片
Recombinant MGEA5/OGA Antibody (Ab115221) - Western Blot All lanes: Recombinant MGEA5/OGA Antibody (Ab115221) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 103 kDa Observed band size: 119 kDa Exposure time: 1.0 s
Recombinant MGEA5/OGA Antibody (Ab115221) - IF/ICC Immunofluorescent analysis of HeLa, using Recombinant MGEA5/OGA Antibody (Ab115221) at 1/50 dilution.
Recombinant MGEA5/OGA Antibody (Ab115221) - IF/ICC Immunofluorescent analysis of HeLa, using Recombinant MGEA5/OGA Antibody (Ab115221) at 1/50 dilution.
ZJ26F0838708
危险品化学品经营许可证（带存储）