概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 此产品的引用文献技术文档和文章引用文献溶液计算器

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为什么选择此级别

EnzymoPure™ 级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

2-8°C储存。低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 6 篇同行评审文献引用。

概述

Butyrl Cholinesterase catalyzes the hydrolysis of a number of choline esters according to the following reaction:

Acylcholine + H2O → choline + acid

It is a tetrameric glycoprotein with four subunits of equal size, each having a molecular weight of 110 kDa. Butyryl cholinesterase hydrolyzes butyrylcholine four times more rapidly than acetylcholine. Unlike acetyl cholinesterase, it does not hydrolyze D-≥-methyl acetylcholine. It is inhibited by 10-5 M physostigmine, numerous organophosphate esters, the carbamate derivates and quaternary ammonium salts.

Specificity

The enzyme is more active with butyryl and propionyl choline than with acetyl choline. Non-choline esters, such as procaine, morphine esters, atropine and cocaine, are susceptible to the action of ButChE.

Composition

ButChE presents a tetrameric structure with equally sized subunits of 110,000 daltons. It is a glycoprotein. The amino acid and carbohydrate composition has been determined based upon a particle weight of 101,000.

Characteristics

Molecular Weight: 440,000

Optimal pH: 6.0–8.0

Extinction Coefficient: E280 = 13.6

Activators: Ca²⁺ and Mg²⁺

Inhibitors: Numerous organophosphate esters, carbamate derivatives and quaternary ammonium salts.

Assay

Method

The reaction velocity is determined by a titrimetric measurement of the acid released during the hydrolysis of acetylcholine. One Unit causes hydrolysis of one micromole of acetylcholine per minute at 25°C and pH 7.4, under the specified conditions.

Reagents

0.02 M Tris⋅HCl buffer, pH 7.4

0.2 M Magnesium chloride

2.2 M Acetylcholine chloride

Titrant: 0.01-0.02 N NaOH, standardized

Enzyme

Dissolve at one mg/ml in reagent grade water.

Procedure

The titration can be measured with either an automatic titrator or with a laboratory pH meter. The reaction vessel should be maintained at 25°C.

Pipette the following into a titration vessel at 25°C

Reagent grade water 7.0 ml

0.2 M Magnesium chloride 3.0 ml

0.02 M Tris⋅HCl buffer, pH 7.4 3.0 ml

Enzyme 0.1-0.5 ml

Adjust pH to 7.5 with titrant. At zero time add 1 ml of 2.2 M acetylcholine and adjust pH to 7.4. Record the amount of standard base required to maintain the pH at 7.4 for 5-6 minutes after a constant rate is achieved.

Calculation

规格

产品名称

丁酰胆碱酯酶来源于马血清

别名

拟胆碱酯酶 | 胆碱脂酶 | 丁酰基, 酰胆碱酰基水解酶

英文别名

规格或纯度

EnzymoPure™, ≥4 units/mg dry weight

生化机理

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters (By similarity).

生物活性

≥4 units/mg dry weight

种属

马(Horse)

Accession #

P81908

预测分子量

440 kDa

CAS编号和信息

9001-08-5

酶学委员会编号

3.1.1.8

分子类型

酶

储存与运输

物理形态

冻干(Lyophilized)

浓度

≥4 units/mg dry weight

储存条件

2-8°C储存

运输条件

低温运输

单位定义

1 unit hydrolyzes 1.0μmol of acetylcholine per minute at 25°C, pH 7.4.

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
F2609352	分析证书	B128570
J2225259	分析证书	B128570
J2225265	分析证书	B128570

技术文档和文章

细胞培养中的培养基和补充剂

目录：技术文章

探索主题:

培养基

Media and Supplements in Cell Culture

目录：技术文章

探索主题:

此产品的引用文献

如何在您的发表作品中引用阿拉丁的产品和网站? 引用文献检索

引用文献

1. haozhi Wang, Jinyu Lai, Xiaohui Xu, Wei Yu, Xinghua Wang. (2023) Combination of gold nanoclusters and silicon quantum dots for ratiometric fluorometry: One system, two mechanisms. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, [PMID:38198882] [10.1016/j.jpba.2023.115940]
2. Ya-Jie Zhang, Lan Guo, Shuai Chen, Yong-Liang Yu, Jian-Hua Wang. (2020) A portable photoacoustic device for facile and sensitive detection of serum alkaline phosphatase activity. ANALYTICA CHIMICA ACTA, [PMID:32222244] [10.1016/j.aca.2020.02.054]
3. Mingfei Wu, Jie Ma, Lijun Ji, Min Wang, Jianfei Han, Zeng Li. (2019) Design, synthesis, and biological evaluation of rutacecarpine derivatives as multitarget-directed ligands for the treatment of Alzheimer's disease. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, [PMID:31136894] [10.1016/j.ejmech.2019.05.055]
4. Conghuan Lin, Huizhi Du. (2024) Interactions between forsythoside E and two cholinesterases at the different conditions: fluorescence sections. Methods and Applications in Fluorescence, 12 (2): (025003). [PMID:38428023] [10.1088/2050-6120/ad2f3a]
5. Zhenpeng Men, Ragini Singh, Bingyuan Zhang, Santosh Kumar. (2025) Seven-Core Tapered Optical Fiber LSPR Biosensor with CMC Structure for Ultra-Sensitive Detection of Paraoxon in Environmental Monitoring. IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT, [10.1109/TIM.2025.3552480]
6. Bing Tan, Qian Wang, Qiaoli Liu, Yimei Wu, Luyao Ban, Yingli Yan, Xiang Li, Guifen Zhu, Jing Fan, Huimin Zhao. (2025) γ-cyclodextrin metal-organic framework-activated gold nanoclusters with water solubility as multi-enzyme mimics for simultaneous detection of pesticide and cholinesterase activity. ANALYTICA CHIMICA ACTA, [PMID:40374245] [10.1016/j.aca.2025.344093]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

📚 按应用领域分类引用

色谱分析（HPLC / GC / TLC）1

查看全部 6 篇引用 →

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查看 EnzymoPure™ 级别指南 →

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丁酰胆碱酯酶 来源于马血清

为什么选择此级别

储存与运输

质量文档

文献证明

概述

Butyrl Cholinesterase catalyzes the hydrolysis of a number of choline esters according to the following reaction:

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

📚 按应用领域分类引用

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丁酰胆碱酯酶来源于马血清