计算溶液所需的质量、体积或浓度。
骨组织番红固绿染液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
软骨组织由软骨细胞和软骨基质组成，软骨组织及其周围的软骨膜构成软骨，软骨根据基质内所含纤维素成分不同分为透明软骨、弹性软骨、纤维软骨。软骨染色方法有很多种，例如甲苯胺蓝法、阿利新蓝法、番红O法等。
骨组织番红固绿染液的染色原理在于嗜碱性的软骨与碱性染料番红O结合呈现红色，嗜酸性的骨和酸性染料固绿结合而呈绿色或蓝色，与呈现红色的软骨对比鲜明，从而将软骨组织与骨组织区分开。番红O是一种结合多阴离子的阳离子染料，其显示软骨是基于阳离子染料与多糖中阴离子基团（硫酸软骨素或硫酸角质素）结合，番红O着色与阴离子的浓度近似成正比关系，间接反映基质中蛋白多糖的含量和分布；当软骨受到损伤时软骨中的糖蛋白会释放出来，使基质成分分布不均匀，从而导致番红O淡染或不着色，通过图像分析软件可对番红O染色的软骨基质进行定量分析。酸性染料固绿则与组织中的嗜酸性成分结合使其呈绿色或蓝色。该试剂仅适用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
| B1511536 | Component | 3×100mL | Storage |
| B1511536A | 固绿染色液 | 100mL | RT |
| B1511536B | 酸性乙醇分化液 | 100mL | RT |
| B1511536C | 番红O染色液 | 100mL | RT |
自备材料：
1、10%福尔马林固定液、脱钙液、蒸馏水、系列乙醇
2、二甲苯或环保脱蜡透明液、中性树胶或环保封片胶
操作步骤(仅供参考)：
1、标本的处理：10%福尔马林固定、脱钙、石蜡切片，二甲苯或脱蜡透明液脱蜡至水。
2、滴加固绿染色液浸染1~5min。
3、入1%盐酸乙醇快速分化10~15s，自来水稍洗。
4、滴加番红O染色液染色2~5min。
5、无水乙醇快速脱水四次，每次3~5s，第四次脱水后镜检，至软骨呈红色，背景应无色。
6、二甲苯或环保脱蜡透明液透明、中性树胶封片、显微镜镜检、图像采集分析。
染色结果：
软骨：红色或橙红色
成骨：绿色
结缔组织：红色
注意事项：
1、需要显示细胞核时，建议采用铁苏木素染色，其着色力强且色调浓。
2、组织在番红O染色液中的时间不宜过长，易与绿色杂合呈紫蓝色。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Cartilage tissue is composed of chondrocytes and cartilage matrix. Cartilage tissue and the surrounding perichondrium constitute cartilage. According to the different fibrous components in the matrix, cartilage is classified into hyaline cartilage, elastic cartilage, and fibrocartilage. There are various staining methods for cartilage, such as toluidine blue staining, alcian blue staining, safranin O staining, etc.
The staining principle of Safranin O‑Fast Green Staining Solution for bone tissue is that basophilic cartilage binds to the basic dye safranin O and appears red, while acidophilic bone binds to the acidic dye fast green and appears green or blue. This forms a sharp contrast with the red‑stained cartilage, thereby distinguishing cartilage tissue from bone tissue. Safranin O is a cationic dye that binds to polyanions. It stains cartilage based on the binding between cationic dye and anionic groups in polysaccharides (chondroitin sulfate or keratan sulfate). The staining intensity of safranin O is approximately proportional to the concentration of anions, indirectly reflecting the content and distribution of proteoglycans in the matrix. When cartilage is injured, glycoproteins in the cartilage are released, resulting in uneven distribution of matrix components, which leads to weak or absent staining by safranin O. Quantitative analysis of safranin O‑stained cartilage matrix can be performed using image analysis software. The acidic dye fast green binds to acidophilic components in the tissue and stains them green or blue. This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| B1511536 | Component | 3×100mL | Storage |
| B1511536A | Fast Green Staining Solution | 100mL | RT |
| B1511536B | Acid Ethanol Differentiation Solution | 100mL | RT |
| B1511536C | Safranin O Staining Solution | 100mL | RT |
Materials Required (User-supplied):
1. 10% Formalin Fixative, Decalcifying Solution, Distilled Water, Graded Ethanol
2. Xylene or Environment-friendly Dewaxing & Clearing Agent, Neutral Balsam or Environment-friendly Mounting Medium
Protocol (For Reference Only):
1. Specimen Processing: Fix in 10% formalin, decalcify, prepare paraffin sections, dewax with xylene or dewaxing & clearing agent, and rehydrate to water.
2. Stain with Fast Green Staining Solution for 1–5 minutes.
3. Differentiate rapidly in 1% Hydrochloric Acid Ethanol for 10–15 seconds, rinse briefly with tap water.
4. Stain with Safranin O Staining Solution for 2–5 minutes.
5. Dehydrate rapidly in anhydrous ethanol four times, 3–5 seconds each time. Examine under microscope after the fourth dehydration: cartilage should appear red, and the background colorless.
6. Clear with xylene or environment-friendly dewaxing & clearing agent, mount with neutral balsam, examine under microscope, and perform image acquisition and analysis.
Staining Results:
Cartilage: Red or orange-red
Osteogenesis: Green
Connective Tissue: Red
Precautions:
1. If nuclear staining is required, iron hematoxylin staining is recommended for strong and intense staining.
2. Prolonged staining in Safranin O Staining Solution should be avoided, as it may mix with green to produce a purplish-blue color.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.
规格
别名
番红O-固绿软骨染色液
英文别名
Bone Tissue Safranin O‑Fast Green Staining Solution
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
Store at room temperature long term (12 months).
英文名称
Safranin-Fast Green Staining Solution for Bone Tissue
储存条件
室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434255 |
分析证书 | B1511536 |
技术文档和文章
溶液计算器
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