计算溶液所需的质量、体积或浓度。
有货
概述
分子量：ca. 70 kDa
Product introduction
Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.
Application
The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. < or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase.
规格
产品名称
荧光素酶 来源于大肠杆菌
英文别名
Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) | firefly luciferase | luciferase (firefly luciferin) | Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing) | firefly luciferin luciferase | Photinus pyralis luciferase
规格或纯度
EnzymoPure™, 重组, 1 mg/ml，＞ 1.0 x 10^10 relative light units (RLU)/ml Specific activity: 1.9 x 10^11 RLU/mg purified protein
CAS编号和信息
61970-00-1
酶学委员会编号
1.13.12.7
分子类型
酶
产品线
生物产品
储存与运输
浓度
1 mg/ml，＞ 1.0 x 10^10 relative light units (RLU)/ml Specific activity: 1.9 x 10^11 RLU/mg purified protein
储存条件
-20°C储存
运输条件
超低温运输
单位定义
One relative light unit (RLU) is defined as the amount of enzyme which emits 1 count of light for 20 s at 30°C and pH 7.8.
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
此产品的引用文献
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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