计算溶液所需的质量、体积或浓度。
BioReagent,for western blot,即用型,用于聚丙烯酰胺凝胶电泳,适用于电泳 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品是阿拉丁最新研发的快速、高灵敏、低背景、无毒无污染的考马斯亮蓝染色液。可用于SDS-PAGE及非变性胶中蛋白质的快速染色。最短染色15min即可观察到纳克级别的蛋白条带,且背景极低,纯水漂洗处理即能进一步降低背景,而无需有毒的脱色液脱色处理。
产品特点
1、快速灵敏——最少仅需染色15min即可观察低至10ng的蛋白条带,适当延长染色时间可进一步提高灵敏度;
2、背景极低——染色结束后即可观察到清晰的蛋白条带,背景极低,使用纯水洗涤即可进一步降低背景染色,提高信噪比;
3、环保无毒——本品使用环保配方,无毒,无刺激性气味,保护实验人员健康。
使用说明
1、电泳结束后,将凝胶置于纯水中漂洗数分钟去除杂质,以降低背景;
2、弃去纯水,加入适量本品,以覆盖凝胶为宜,室温下于水平摇床上染色10~15 min。如蛋白量较低,可适当延长染色时间,实际染色时间可根据条带显现程度决定。染色结束后,弃去染色液,加入纯水洗涤去除残留染液,即可观察结果;
3、如需进一步降低染色背景,可每隔20min更换纯水进行漂洗,重复4~5次即可得到背景极低的凝胶。此外,如将染色后的凝胶在纯水中浸泡过夜,可以达到更好的去背景效果,从而获得更清晰的蛋白条带。
注意事项
1、本试剂含挥发性物质,请注意密封以避免失效;
2、如染色后蛋白条带不明显,则说明蛋白含量过低。建议延长染色时间或延长纯水洗涤时间,然后再进行观察。同时,点样时可加入阳性对照,以检测染色效果;
3、由于SDS会干扰考马斯亮蓝与蛋白质的结合,可以先使用加热后的纯水漂洗凝胶,去除胶中残留的SDS再进行染色,效果更佳;
4、为了您的安全和健康,请穿实验服并戴一次性手套操作;
5、本产品仅限科研使用。
This product is a newly developed rapid, highly sensitive, low-background, non-toxic, and environmentally friendly Coomassie Blue staining solution from Aladdin. It is suitable for rapid protein staining in SDS-PAGE and non-denaturing gels. Protein bands at the nanogram level can be visualized after just 15 minutes of staining, with extremely low background. Further background reduction can be achieved by rinsing with pure water, eliminating the need for toxic destaining solutions.
Rapid and Sensitive: Detect protein bands as low as 10 ng with just 15 minutes of staining. Sensitivity can be further improved by extending staining time.
Low Background: Clear protein bands are visible immediately after staining. Rinsing with pure water further reduces background and improves signal-to-noise ratio.
Environmentally Friendly: Non-toxic formula with no irritating odor, ensuring user safety.
After electrophoresis, rinse the gel in pure water for a few minutes to remove impurities and reduce background.
Discard the water and add sufficient staining solution to cover the gel. Stain on a horizontal shaker at room temperature for 10–15 minutes. For low-abundance proteins, extend the staining time as needed. After staining, discard the solution and rinse with pure water to remove residual dye.
For further background reduction, replace the pure water every 20 minutes and repeat 4–5 times. Alternatively, incubating the stained gel in pure water overnight can achieve even lower background and clearer bands.
This reagent contains volatile compounds. Ensure tight sealing to avoid loss of efficacy.
If protein bands are faint after staining, it may indicate low protein content. Extend staining or rinsing time. Include a positive control during sample loading to validate staining performance.
SDS can interfere with Coomassie Blue-protein binding. Pre-rinse the gel with warm pure water to remove residual SDS for improved staining results.
Wear a lab coat and disposable gloves for safety.
For research use only.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C1492039 | |
| 分析证书 | C1492039 | |
| 分析证书 | C1492039 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Suitable for electrophoresis 级别指南 → 查看 BioReagent 级别指南 → 查看 Ready-to-use 级别指南 → 查看 for PAGE 级别指南 → 查看 for Western blot 级别指南 →