计算溶液所需的质量、体积或浓度。
刚果红染色液(1%)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
淀粉样物质是一种无固定形状的细胞外嗜酸性物质，可存在于不同的组织、器官，导致的疾病称为淀粉样变；淀粉样物质主要是由蛋白质构成，该蛋白大部分排列成反向的β-折叠层结构。在电子显微镜下淀粉样物质呈原纤维排列，病例材料中为大量细胞外的不分支的细丝，大多随机排列；用于识别淀粉样物质的组织学方法有甲紫染色、刚果红染色、偏振光显微镜观察等。目前研究发现传统的甲紫染色法灵敏度低、特异性差，经典的而且有效的方法是刚果红染色，1922年Bennhold发现了刚果红可以用于活体内淀粉样物质的鉴别，并应用到组织切片。
刚果红染色液 (1%) 染色原理是淀粉样物质对刚果红比其他的组织结构具有更大的亲和力，其羟基与刚果红的氨基结合，从而使淀粉样物质染成红色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
10%中性福尔马林固定液、蒸馏水、系列乙醇
操作步骤(仅供参考)：
1、常规固定，常采用10%中性福尔马林固定液，常规脱水包埋。
2、切片厚度4μm，常规二甲苯或脱蜡透明液脱蜡至水。
3、入刚果红染色液(1%)浸染30min。
4、进行后续染色步骤。
染色结果：
淀粉样物质：红色
注: 在偏光显微镜下，淀粉样物质呈黄绿色的双折光。
注意事项：
1、切片脱蜡应尽量干净，否则影响染色效果。
2、刚果红染色液染色时尽量采用浸染，如果滴染，应置于湿盒防止溶液挥发。
3、分化时间较短，胶原纤维也被染成红色；分化过度，淀粉样物质也被脱色；如果脱色过度，可以将切片清洗后重新用刚果红染色液浸染。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、试剂开封后请尽快使用，以防影响后续实验效果。
Amyloid is an amorphous, extracellular, eosinophilic substance that can be present in various tissues and organs, and the disease it causes is known as amyloidosis. Amyloid is mainly composed of proteins, most of which are arranged in an antiparallel β‑pleated sheet structure. Under an electron microscope, amyloid appears as fibrils; in pathological specimens, it consists of abundant extracellular non‑branching filaments, mostly randomly arranged. Histological methods for identifying amyloid include methyl violet staining, Congo red staining, and observation under polarized light microscopy. Studies have shown that the traditional methyl violet staining method has low sensitivity and poor specificity, while the classic and effective method is Congo red staining. In 1922, Bennhold discovered that Congo red could be used to identify amyloid in vivo and applied it to tissue sections.
The staining principle of Congo Red Staining Solution (1%) is that amyloid has a greater affinity for Congo red than other tissue structures. Its hydroxyl groups combine with the amino groups of Congo red, thereby staining amyloid red. This reagent is for research use only. Not for clinical diagnosis or other purposes.
Materials Required:
10% neutral formalin fixative, distilled water, graded ethanol series
Protocol (for reference only):
1. Routine fixation, usually with 10% neutral formalin fixative, followed by routine dehydration and embedding.
2. Cut sections at 4 μm thickness. Deparaffinize routinely in xylene or dewaxing agent to water.
3. Stain in Congo Red Staining Solution (1%) for 30 minutes.
4. Proceed with subsequent staining steps.
Staining Results:
Amyloid: Red
Note: Under polarized light microscopy, amyloid shows yellow-green birefringence.
Precautions:
1. Section deparaffinization should be thorough, otherwise staining quality will be affected.
2. Prefer immersion staining with Congo Red Staining Solution. If using drop staining, sections should be kept in a moist chamber to prevent evaporation.
3. Short differentiation time may also stain collagen fibers red; excessive differentiation will decolorize amyloid.
4. If over-decolorized, sections may be rinsed and restained with Congo Red Staining Solution.
5. For your safety and health, wear laboratory coat and disposable gloves during operation.
6. Use the reagent promptly after opening to avoid affecting experimental results.
规格
别名
淀粉样物质刚果红染色液 | 淀粉样蛋白刚果红染色液 | 淀粉样蛋白染色液
英文别名
Congo Red Stain Solution
规格或纯度
BioReagent,生物染色剂,用于显微镜,1%
稳定性与储存
Store at room temperature long term (12 months).
英文名称
Congo Red Staining Solution (1%)
储存条件
室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0737403 |
分析证书 | C1516057 |
| ZJ26F0434131 |
分析证书 | C1516057 |
技术文档和文章
溶液计算器
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