计算溶液所需的质量、体积或浓度。
BioReagent,无菌,无菌过滤,用于细胞培养,通过内毒素测试 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Dulbecco的改良Eagle培养基—DMEM(Dulbecco's Modified Eagle Medium)是一种广泛使用的基础培养基,适用于多种哺乳动物细胞培养,包括原代成纤维细胞,神经元,神经胶质细胞,HUVEC和平滑肌细胞,以及HeLa,293,Cos-7和PC-12等细胞系。DMEM是在MEM培养基的基础上研制的,与MEM培养基相比,氨基酸的含量增加了2倍, 维生素增加了4倍,同时还增加了非必需氨基酸、微量铁离子以及丙酮酸钠。
DMEM培养基最初设计为葡萄糖含量1000mg/L的低糖型,后来又发展出葡萄糖含量为 4500mg/L的高糖型,现已广泛应用于各种细胞的培养。不含葡萄糖的DMEM培养基可以根据研究需求,自行调节葡萄糖的含量,使用便捷。
丙酮酸钠可以作为细胞培养中的替代碳源,在葡萄糖不足的情况下,细胞也可以代谢丙酮酸钠。不含丙酮酸钠的培养基,可以根据研究需求,自行调整丙酮酸钠的含量,以控制细胞代谢的碳源。
产品含有:酚红、L-谷氨酰胺。
不含有:D-葡萄糖、丙酮酸钠、HEPES。
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for the culture of a variety of mammalian cells, including primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos‑7 and PC‑12. Developed on the basis of MEM medium, DMEM contains twice the concentration of amino acids and four times the concentration of vitamins compared with MEM. It is additionally supplemented with non‑essential amino acids, trace iron ions and sodium pyruvate.
DMEM was originally formulated as a low‑glucose version with a glucose concentration of 1000 mg/L. A high‑glucose variant containing 4500 mg/L glucose was subsequently developed and is now widely adopted for diverse cell culture applications. Glucose‑free DMEM allows users to adjust glucose concentrations according to research requirements, offering flexible usage.
Sodium pyruvate serves as an alternative carbon source in cell culture, enabling cellular metabolism even under glucose‑deprived conditions. Sodium pyruvate‑free medium permits researchers to modulate sodium pyruvate levels to control carbon availability for cellular metabolism.
Contains: Phenol Red, L‑Glutamine.
Does not contain: D‑Glucose, Sodium Pyruvate, HEPES.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | D1372065 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
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