DMEM/F-12培养基 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12)是DMEM培养基和Ham's F-12培养基的1:1混合物，是在DMEM培养基的基础上，添加F-12培养基中更为丰富的营养成分，含有多种微量元素。Dulbecco改良Eagle培养基（DMEM）是改良的Eagle基本培养基（BME），氨基酸和维生素浓度是BME的四倍。Ham's F-12以Ham's F-10培养基为基础，显著提高了胆碱、肌醇、腐胺和几种氨基酸的浓度。DMEM/F-12被广泛用于支持多种哺乳动物细胞的生长，包括MDCK、神经胶质细胞、成纤维细胞、人内皮细胞和大鼠的成纤维细胞等。同时，DMEM/F12常作为开发无血清培养基的基础，也适用于低血清含量下哺乳动物细胞的培养以及克隆密度培养。
本产品含有：D-葡萄糖、酚红、L-谷氨酰胺、丙酮酸钠。
不含有：HEPES。

DMEM/F-12 Medium (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a 1:1 mixture of DMEM and Ham's F-12 medium. It is based on DMEM but supplemented with the richer nutrient components of F-12 medium, including various trace elements. Dulbecco's Modified Eagle Medium (DMEM) is a modification of Eagle's Basal Medium (BME), with amino acid and vitamin concentrations four times those of BME. Ham's F-12, developed from Ham's F-10 medium, significantly increases the concentrations of choline, inositol, putrescine, and several amino acids. DMEM/F-12 is widely used to support the growth of various mammalian cells, including MDCK cells, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. It also frequently serves as a basal medium for developing serum-free media and is suitable for mammalian cell culture under low-serum conditions as well as clonal-density culture.

This product contains: D-glucose, phenol red, L-glutamine, and sodium pyruvate.

It does not contain: HEPES.

规格

别名

DMEM/F-12 (1:1) 培养基含酚红和 L-谷氨酰胺,不含 HEPES

英文别名

DMEM/F-12

规格或纯度

无菌过滤, BioReagent, 通过内毒素测试, 用于细胞培养, 无菌, 含D-葡萄糖、酚红、L-谷氨酰胺、丙酮酸钠，不含HEPES

稳定性与储存

Store at 2-8℃ long term (12 months). Store in the dark.

英文名称

DMEM/F-12 Medium

储存条件

2-8°C储存,避光

运输条件

低温运输

产品线

生物产品

名称和识别符

分子类型

培养基

高级数据

化学和物理性质

敏感性

Light-sensitive

质检证书(CoA,COO,BSE/TSE 和分析图谱)

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批号(Lot Number)	证书类型	货号
ZJ25F0723864	分析证书	D1372080

技术文档和文章

d1372080 dmemf 12 培养基中文说明书.pdf

无菌过滤

目录：阿拉丁试剂的规格、级别和纯度

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无菌过滤

Sterile-Filtered

目录：阿拉丁试剂的规格、级别和纯度

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Sterile-Filtered

通过内毒素测试试剂

目录：阿拉丁试剂的规格、级别和纯度

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内毒素重组蛋白分子诊断通过内毒素测试细胞治疗抗体药物制备免疫学研究

Endotoxin-tested Reagents

目录：阿拉丁试剂的规格、级别和纯度

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endotoxin Recombinant proteins Molecular Diagnostics Endotoxin-tested Cell therapy Antibody drug preparation Immunological research

用于细胞培养

目录：阿拉丁试剂的规格、级别和纯度

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用于细胞培养

For Cell Culture

目录：阿拉丁试剂的规格、级别和纯度

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For Cell Culture

DMEM培养基

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DMEM培养基

DMEM Culture Medium

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DMEM Culture Medium

动物细胞培养基常见问题

目录：常见问题(FAQs)

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动物细胞培养基

FAQs on Animal Cell Culture Media

目录：常见问题(FAQs)

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Animal Cell Culture Media

EdU染色实验的基本原理和实验流程

目录：实验方案（Protocols）

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胸腺嘧啶核苷 EdU染色

EdU Staining: Basic Principle and Experimental Workflow

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Thymidine nucleoside EdU staining

骨髓间充质干细胞分离培养及诱导分化为破骨细胞实验操作流程

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骨髓间充质干细胞

Isolation, Culture, and Induced Differentiation of Bone Marrow Mesenchymal Stem Cells into Osteoclasts: Experimental Procedure

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Bone Marrow Mesenchymal Stem Cells

108种实验室常见的细胞系

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细胞系

108 common laboratory cell lines

目录：技术文章

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Cell line

培养基体系的分类、实验定位与选型逻辑

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微生物培养基细胞培养基选择培养基鉴别培养基真菌培养基酵母培养基无血清培养基细胞系专用培养基培养基选型实验质控

Classification, Experimental Positioning, and Selection Logic of Culture Media Systems

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microbial culture medium cell culture medium selective medium differential medium fungal medium yeast medium serum-free medium cell line-specific medium medium selection experimental quality control

常用哺乳动物细胞培养基的配方特征、适用细胞与选择策略

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葡萄糖细胞培养基无血清培养基 RPMI 1640 DMEM MEM IMDM HEPES L-谷氨酰胺丙酮酸钠完全培养基

Formulation Characteristics, Applicable Cell Types, and Selection Strategies for Common Mammalian Cell Culture Media

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Glucose cell culture medium serum-free medium RPMI 1640 DMEM MEM IMDM HEPES L-glutamine sodium pyruvate complete medium

溶液计算器