计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血流中D-乳酸的浓度通常是纳摩尔级,由肠源或甲基乙二醛途径产生。在哺乳动物中,D-乳酸代谢需要D-乳酸氢化酶且代谢缓慢,因此血液浓度水平增加可导致酸血症和酸中毒。D-乳酸酸中毒的严重程度可能与神经毒性症状有关。体内D-乳酸显著积累也可能与代谢和排泄受损有关。
应用
D-乳酸比色检测试剂盒用于检测产生的乳酸的立体特异性。
适用性
适用于血清、血浆、细胞、培养物和发酵培养基样品。
原理
在该检测中,D-乳酸被D-乳酸氢化酶特异性氧化,产生与之成比例、可在450 nm处进行检测的比色产物。样品中D-乳酸的有效浓度范围是0.1-10 mM。
制备说明
外形
D-Lactate, typically present in the bloodstream at nanomolar concentrations, is produced by an intestinal source or via the methylglyoxal pathway. In mammals, D-Lactate metabolism requires D-Lactate hydrogenase and is metabolized slowly, thus an increase in blood concentration levels can lead to acidemia and acidosis. The severity of this D-lactic acidosis can be associated with neurotoxic symptoms. Significant D-Lactate accumulations in the body can also be related to impaired metabolism and excretion.D-Lactate Colorimetric Assay kit has been used to determine the stereospecificity of lactate produced.Suitability: Suitable for use with samples of serum, plasma, cells, culture and fermentation media.Principle: In this assay, D-Lactate is specifically oxidized by D-Lactate hydrogenase and generates a proportional colorimetric product measured at 450 nm. The useful concentration range in samples is 0.1-10 mM D-Lactate.
H318: 造成严重的眼睛损伤
H412: 对水生生物有害并具有长期持续影响
P273: 避免释放到环境中。
P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。
P305+P351+P338: 如进入眼睛:用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出,取出隐形眼镜。继续冲洗。