Dihydrofluorescein diacetate is a fluorescent probe used to measure oxidative stress in cell-free systems and cellular models. It is an excellent fluorescent probe for a wide range of cell-based studies. As it exhibits high reactivity toward specific oxidizing species, it is widely applied to detect intracellular oxidants. Dihydrofluorescein diacetate displays linearly structured fluorescence consistent with mitochondria in reoxygenated endothelial cells, and can detect mitochondrial reactive oxygen species (ROS). Its fluorescence appears as sharp, well-defined filamentous structures, which fully match mitochondria stained with TMRM. The probe penetrates mitochondria and reacts with ROS released in the mitochondrial matrix. Dihydrofluorescein diacetate can serve as a reliable quantitative approach to evaluate the oxidative potential of nanoparticle-treated cells.
规格
规格或纯度
BioReagent,生物染色剂,适用于荧光分析,用于显微镜,≥97%
稳定性与储存
Store at -20℃ long term (12 months). Store in the dark.
危险品化学品经营许可证（带存储）