储存缓冲液： 1 mM Sodium citrate, pH6.5
eGFP mRNA（enhanced Green Fluorescent Protein）编码一种绿色荧光蛋白，该蛋白从水母（Aequorea victoria）中分离，其最大激发 / 发射光波长分别为 488nm/509nm，转染到细胞中可表达出增强的绿色荧光，可作为对照研究靶基因在哺乳动物细胞中的转染和表达。 本产品通过引入 N1-Me-Pseudo UTP 替换天然 UTP，降低 mRNA 在哺乳动物细胞中的免疫原性，增强 mRNA 的稳定性。同时，本产品还是模拟具有 5’Cap 1 结构和 3’poly (A) 尾巴的成熟 mRNA，是使用各种测定法研究转染和表达的理想选择。
产品属性
| 产品浓度： | 1mg/mL |
| 储存溶液： | 1 mM 柠檬酸钠，pH6.5 |
| mRNA 序列全长： | 1073nt |
| 完整性： | ≥85% |
| 产品纯度： | mRNA 电泳条带单一 |
| 转染效率： | 293T 细胞转染 |
产品应用
eGFP-mRNA 作为报告 mRNA，是监测和优化转染效率的理想选择。
- 对照品：良好的转染对照品；
- 指示剂：可以和目的序列融合，直观的观察目的蛋白是否表达成功；
- 药物递送：eGFP mRNA 可以用于评估药物递送系统的效果；
- 体内成像：eGFP mRNA 也用于体内成像研究。
运输和储存
本产品随干冰运输，可在 -20℃ ~-80℃ 保存两年。
注意事项
- 可以室温溶解，溶解后宜存放于冰盒内或冰浴上，使用过程小心防止 RNase 污染降解，使用完毕后宜立即置于 -20℃ ~-80℃ 保存。
- 本产品仅限科学研究用，不得用于临床诊断或治疗，不得用于食品或药品。
- 为了您的安全和健康，请穿实验服并戴一次性手套操作。
Storage buffer: 1 mM Sodium citrate, pH 6.5
eGFP mRNA (enhanced Green Fluorescent Protein) encodes a green fluorescent protein, which is isolated from jellyfish (Aequorea victoria). Its maximum excitation/emission wavelengths are 488nm and 509nm respectively. When transfected into cells, it can express enhanced green fluorescence and can be used as a control to study the transfection and expression of target genes in mammalian cells. This product reduces the immunogenicity of mRNA in mammalian cells and enhances the stability of mRNA by introducing N1-Me-Pseudo UTP to replace natural UTP. Meanwhile, this product also mimics mature mRNA with a 5'Cap 1 structure and a 3'poly(A) tail, making it an ideal choice for studying transfection and expression using various assay methods.
Product Attributes
| Product Concentration: | 1mg/mL |
| Storage Solution: | 1 mM Sodium Citrate, pH6.5 |
| Full Length of mRNA Sequence: | 1073nt |
| Integrity: | ≥85% |
| Product Purity: | Single mRNA Electrophoresis Band |
| Transfection Efficiency: | 293T Cell Transfection |
Product Applications
eGFP-mRNA, as a reporter mRNA, is an ideal choice for monitoring and optimizing transfection efficiency.
- Control: A good transfection control;
- Indicator: It can be fused with the target sequence to visually observe whether the target protein is successfully expressed;
- Drug Delivery: eGFP mRNA can be used to evaluate the effect of drug delivery systems;
- In Vivo Imaging: eGFP mRNA is also used in in vivo imaging studies.
Transportation and Storage
This product is transported with dry ice and can be stored at -20℃~-80℃ for two years.
Precautions
- It can be dissolved at room temperature. After dissolution, it should be stored in an ice box or on an ice bath. During use, be careful to prevent RNase contamination and degradation. After use, it should be immediately stored at -20℃~-80℃.
- This product is for scientific research use only, and shall not be used for clinical diagnosis or treatment, nor for food or drugs.
- For your safety and health, please wear a lab coat and disposable gloves during operation.
危险品化学品经营许可证（带存储）