ECL Universal Chemiluminescence Kit (Femto-grade) is a non-radioactive chemiluminescence system designed for detecting the activity of horseradish peroxidase (HRP) conjugated to proteins bound on PVDF or NC membranes. This product includes an enhanced luminol substrate and a stable peroxide solution, enabling the detection of trace amounts of protein as low as the femto-gram level. With a broad detection range and high sensitivity, it is suitable for most conventional Western Blot chemiluminescence detection applications.
Component List
E1491293
Component
100 mL
500 mL
Storage
E1491293A
E-ECL Substrate
50 mL
250 mL
2-8℃. Store in the dark.
E1491293B
E-ECL Oxidant
50 mL
250 mL
2-8℃. Store in the dark.
Features
High Sensitivity – Capable of rapidly detecting proteins at femto-gram levels, outperforming other high-end products in the same category.
High Cost-Effectiveness – Offers exceptional value compared to other chemiluminescence substrates of similar grade.
Instructions for Use
Remove the transfer membrane from the washing buffer and drain excess liquid, ensuring the membrane remains moist.
Mix E-ECL Substrate and E-ECL Oxidant in equal volumes to prepare the chemiluminescence working solution (approximately 0.1 mL per square centimeter of membrane).
Evenly apply the chemiluminescence working solution onto the membrane and incubate for 1–2 minutes. For highly abundant target proteins, avoid letting the working solution remain stationary. Instead, place the membrane on a shaker for 1–2 minutes to prevent localized depletion of reactants, which may affect the linear range of detection signals.
Remove the membrane and drain excess working solution. Wrap the membrane in a transparent protective cover.
X-ray Film Exposure: In a darkroom, expose the membrane to X-ray film. Exposure times may range from a few seconds to several minutes. Develop the film and, if necessary, adjust the exposure time for a second exposure based on the initial results. CCD Imager: The membrane can be directly imaged without a protective cover. Follow the instrument’s instructions for imaging.
The membrane can be stripped and reused for multiple hybridizations.
Precautions
Avoid strong light exposure to E-ECL Substrate and the working solution, as it may reduce sensitivity.
Due to the high sensitivity of the ECL solution, avoid using excessively high antibody concentrations to prevent increased background signals.
Sodium azide (NaN₃) inhibits HRP activity. Avoid using NaN₃ for the storage or recycling of secondary antibodies.
For safety and health reasons, wear lab coats and disposable gloves during operation.
This product is intended for research use only.
规格
别名
ECL化学发光显色试剂盒
英文别名
ECL Detection Reagent
规格或纯度
BioReagent, 用于化学发光, for western blot
英文名称
ECL Efficient Light Chemiluminescence Kit
应用
WB
敏感性
Light-sensitive
储存与运输
储存条件
2-8°C储存,避光
运输条件
低温运输
稳定性与储存
Store at 2-8℃ long term (12 months). Store in the dark.
ZJ25F1129823
危险品化学品经营许可证（带存储）