ECL Basic Chemiluminescence Detection Kit (Pico-grade) is a non-radioactive chemiluminescence system designed for detecting the activity of horseradish peroxidase (HRP) conjugated to membrane-bound proteins. This product contains an enhanced luminol substrate and a stable peroxide solution, enabling the detection of trace amounts of protein as low as the pico-gram level. It is suitable for routine Western Blot chemiluminescence detection.
Component List
Component
100 mL
500 mL
Storage
E1491297A
ECL PicoLight Substrate
50 mL
250 mL
2-8℃. Store in the dark.
E1491297B
ECL PicoLight Oxidant
50 mL
250 mL
2-8℃. Store in the dark.
Features
High Sensitivity – Capable of rapidly detecting proteins at pico-gram levels, outperforming other high-end products in the same category.
High Cost-Effectiveness – Offers exceptional value compared to other chemiluminescence substrates of similar grade.
Instructions for Use
Remove the transfer membrane from the washing buffer and drain any excess liquid, keeping the membrane moist.
Mix PicoLight ECL Substrate and Oxidant in equal volumes to prepare the chemiluminescence working solution (approximately 0.1 mL per square centimeter of membrane).
Apply the working solution evenly onto the membrane and incubate for 1–2 minutes. For strong target protein signals, avoid letting the working solution remain static. Gently agitate the membrane on a shaker for 1–2 minutes to prevent local depletion of reactants, which may affect the linear detection range.
Remove the membrane and drain any excess working solution. Wrap it in a transparent protective cover.
X-ray Film Exposure: In a darkroom, expose the membrane to X-ray film. Exposure times may vary from seconds to minutes. Develop the film and adjust exposure time for repeat imaging if necessary. CCD Imager: The membrane can be directly imaged without a protective cover. Follow the manufacturer’s instructions for image capture.
The membrane can be stripped and reprobed for multiple hybridizations.
Precautions
Protect PicoLight ECL Substrate and the working solution from strong light to avoid reduced sensitivity.
Due to the high sensitivity of the solution, avoid using overly concentrated antibodies to prevent high background signals.
Sodium azide (NaN₃) inhibits HRP activity. Do not use NaN₃ for storing or reusing secondary antibodies.
Wear a lab coat and disposable gloves for personal safety.
This product is for research use only.
规格
别名
ECL化学发光显色试剂盒
英文别名
ECL Detection Reagent
规格或纯度
BioReagent, 用于化学发光, for western blot
英文名称
ECL Pico Light Chemiluminescence Kit
应用
WB
敏感性
Light-sensitive
储存与运输
储存条件
2-8°C储存,避光
运输条件
低温运输
稳定性与储存
Store at 2-8℃ long term (12 months). Store in the dark.
ZJ25F0927270
危险品化学品经营许可证（带存储）